Institut de Chimie des Substances Naturelles ICSN, Centre National de la Recherche Scientifique, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
Department of Pharmacognosy & Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece.
Molecules. 2019 Jun 15;24(12):2239. doi: 10.3390/molecules24122239.
The strain CA-244599 isolated from the Comoros islands was submitted to liquid-state fermentation coupled to in situ solid-phase extraction with amberlite XAD-16 resin. Elution of the trapped compounds on the resin beads by ethyl acetate afforded seven metabolites, osmanicin (), streptazolin (), streptazone C (), streptazone B (), streptenol C (), nocardamine () and desmethylenylnocardamine (). Osmanicin () is a newly reported unusual scaffold combining streptazolin () and streptazone C () through a Diels-Alder type reaction. Experimental evidence excluded the spontaneous formation of from and . The isolated compounds were evaluated for their ability to inhibit elastase using normal human diploid fibroblasts. Compound exhibited the most potent activity with an IC of 3.7 μM.
从科摩罗群岛分离得到的菌株 CA-244599 经过液体发酵与 Amberlite XAD-16 树脂原位固相萃取相结合。用乙酸乙酯洗脱树脂珠上捕获的化合物，得到了七种代谢产物：奥斯米丁（）、链氮霉素（）、链氮霉素 C（）、链氮霉素 B（）、链烯醇 C（）、诺卡胺（）和去亚甲基诺卡胺（）。奥斯米丁（）是一种新报道的不寻常支架，通过 Diels-Alder 型反应将链氮霉素（）和链氮霉素 C（）结合在一起。实验证据排除了从和自发形成的可能性。分离得到的化合物被评估为抑制弹性蛋白酶的能力，使用正常的人二倍体成纤维细胞。化合物表现出最强的活性，IC 为 3.7 μM。
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