Cheng Yih-Lin, Chen Yi-Wen, Wang Kan, Shie Ming-You
Department of Mechanical Engineering, National Taiwan University of Science and Technology, Taipei City, Taiwan.
J Mater Chem B. 2016 Oct 14;4(38):6307-6315. doi: 10.1039/c6tb01377e. Epub 2016 Sep 12.
The purpose of this study is to develop PCL scaffolds using stereolithography technology and induced modifications using a poly dopamine (PDA)-coated/HA precipitate to stimulate human mesenchymal stem cells (hMSCs). The chemical composition and surface properties of HA/PDA/PLA were characterized by XPS. HA/PDA/PLA modulated hMSCs responses in several ways. The extracellular matrix (collagen and fibronectin) adsorption was significantly higher on the substrates with the highest amount of PDA coating than on pure PCL. Increased focal adhesion kinase (FAK) levels and enhanced cell attachment were observed upon an increase in PDA content. The proliferation, alkaline phosphatase, osteogenesis-related proteins (OPN and BSP), and angiogenesis-related protein (vWF and ang-1) secretion of hMSCs were significantly stimulated when the PDA-coated concentration was increased. The PDA-coated/HA precipitate increases osteogenesis and angiogenesis of hMSCs cultured with a PCL scaffold. Our results demonstrate that this simple, bio-inspired surface modification of the organic PCL scaffold using PDA is a very promising tool for regulating cell behaviour, and may serve as an effective stem cell delivery carrier for bone tissue engineering.
本研究的目的是使用立体光刻技术制备聚己内酯（PCL）支架，并通过聚多巴胺（PDA）包被/羟基磷灰石（HA）沉淀诱导修饰来刺激人间充质干细胞（hMSCs）。采用X射线光电子能谱（XPS）对HA/PDA/聚乳酸（PLA）的化学成分和表面性质进行了表征。HA/PDA/PLA以多种方式调节hMSCs的反应。与纯PCL相比，PDA包被量最高的底物上细胞外基质（胶原蛋白和纤连蛋白）的吸附显著更高。随着PDA含量的增加，观察到粘着斑激酶（FAK）水平升高和细胞附着增强。当PDA包被浓度增加时，hMSCs的增殖、碱性磷酸酶、成骨相关蛋白（骨桥蛋白和骨涎蛋白）以及血管生成相关蛋白（血管性血友病因子和血管生成素-1）的分泌均受到显著刺激。PDA包被/HA沉淀增加了与PCL支架共培养的hMSCs的成骨和血管生成。我们的结果表明，这种使用PDA对有机PCL支架进行的简单、仿生表面修饰是一种非常有前景的调节细胞行为的工具，并且可能作为骨组织工程的有效干细胞递送载体。
