活性氧改变海洋来源真菌 F31-1 的代谢物图谱。

Reactive oxygen species altering the metabolite profile of the marine-derived fungus F31-1.

机构信息

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.

School of Bioscience & Bioengineering, South China University of Technology, Guangzhou, China.

出版信息

Nat Prod Res. 2021 Jan;35(1):41-48. doi: 10.1080/14786419.2019.1611816. Epub 2019 Jun 19.

DOI:10.1080/14786419.2019.1611816

PMID:31215239

Abstract

To investigate the influence of reactive oxygen species (ROS) on the secondary metabolites of the marine-derived fungus F31-1, hydrogen peroxide (HO) was added to the GPY culture medium. The HPLC chromatogram of the EtOAc extract of the culture broth was distinct from that of the HO free GPY medium. Further study of the metabolites in the GPY medium with HO resulted in the discovery of eight known compounds. Among them, (22)-5α, 8α-epidioxyergosta-6, 22-dien-3-ol () and ergosta-4,6,8(14),22-tetraene-3-one () were present in the highest concentration, while ergosterol and diketopiperazines are abundant in the HO free medium. Additionally, a new compound, dichocetide D () containing a chlorine element and a known ergosterol () were isolated from the HO free medium. (22)-5α, 8α-epidioxyergosta-6, 22-dien-3-ol () exhibited moderate cytotoxic activity against human prostate cancer cell line LNCaP-C4-2B.

摘要

为了研究活性氧（ROS）对海洋来源真菌 F31-1 次生代谢产物的影响，向 GPY 培养基中添加了过氧化氢（HO）。培养物中 EtOAc 提取物的 HPLC 图谱与不含 HO 的 GPY 培养基的图谱明显不同。进一步研究含 HO 的 GPY 培养基中的代谢产物，发现了 8 种已知化合物。其中，（22）-5α,8α-表环氧麦角甾-6,22-二烯-3-醇（）和麦角甾-4,6,8(14),22-四烯-3-酮（）的浓度最高，而不含 HO 的培养基中则富含麦角甾醇和二酮哌嗪。此外，从不含 HO 的培养基中分离出一种含有氯元素的新化合物二氢乙酰二肽 D（）和一种已知的麦角甾醇（）。（22）-5α,8α-表环氧麦角甾-6,22-二烯-3-醇（）对人前列腺癌细胞系 LNCaP-C4-2B 具有中等的细胞毒性活性。