School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China.
Mar Drugs. 2017 Nov 1;15(11):339. doi: 10.3390/md15110339.
By adding l-tryptophan and l-phenylalanine to GPY medium, twenty-eight compounds, including amides, polyketides, a sesquiterpenoid, a diterpenoid, a meroterpenoid, diketopiperazines, β-carbolines, fumiquinazolines, and indole alkaloids, were discovered from the marine-derived fungus F31-1, demonstrating the tremendous biosynthetic potential of this fungal strain. Among these compounds, four amides dichotomocejs A-D (-), one polyketide dichocetide A (), and two diketopiperazines dichocerazines A-B ( and ) are new. The structures of these new compounds were determined by interpreting detailed spectroscopic data as well as calculating optical rotation values and ECD spectra. Obviously, can effectively use an amino acid-directed strategy to enhance the production of nitrogen-containing compounds. Dichotomocej A () displayed moderate cytotoxicity against the human rhabdomyosarcoma cell line RD with an IC value of 39.1 µM, and pityriacitrin () showed moderate cytotoxicity against the human colon carcinoma cell line HCT116 with an IC value of 35.1 µM.
通过在 GPY 培养基中添加 l-色氨酸和 l-苯丙氨酸，从海洋来源的真菌 F31-1 中发现了 28 种化合物，包括酰胺、聚酮、倍半萜、二萜、杂二萜、二酮哌嗪、β-咔啉、呋咱喹啉和吲哚生物碱，证明了该真菌菌株具有巨大的生物合成潜力。在这些化合物中，四个酰胺化合物 dichotomocejs A-D（-），一个聚酮化合物 dichocetide A（）和两个二酮哌嗪化合物 dichocerazines A-B（和）是新的。这些新化合物的结构通过解释详细的光谱数据以及计算旋光值和 ECD 光谱来确定。显然，能够有效地利用氨基酸定向策略来增强含氮化合物的产生。dichotomocej A（）对人横纹肌肉瘤细胞系 RD 显示出中等的细胞毒性，IC 值为 39.1 µM，而 pityriacitrin（）对人结肠癌细胞系 HCT116 显示出中等的细胞毒性，IC 值为 35.1 µM。
