Ai Guihai, Meng Meng, Wang Lian, Shao Xiaowen, Li Yue, Cheng Jiajing, Tong Xiaowen, Cheng Zhongping
Department of Gynaecology and Obstetrics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University Shanghai, China.
Department of Prenatal Diagnosis and Fetal Medicine, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai, China.
Am J Transl Res. 2019 May 15;11(5):3081-3091. eCollection 2019.
microRNAs play important roles in proliferation and differentiation of stem cells, but mechanisms by which microRNAs regulate osteogenic or adipogenic differentiation of adipose stem cells (ASCs) are still poorly understood. In the present study, results showed up-regulation of microRNA-196a was able to promote the osteogenic differentiation of ACSs, but down-regulation of microRNA-196a induced adipogenic differentiation. Further investigation indicated microRNA-196a could regulate Wnt signaling pathway to affect osteogenic or adipogenic differentiation of ASCs, addition of Wnt agonist 1 was able to reverse the down-regulated osteogenic differentiation of ASCs caused by microRNA-196a deficiency and inhibition of Wnt signaling pathway with XAV939 promoted the adipogenic differentiation of ASCs. Taken together, microRNA-196a may regulate Wnt signaling pathway to promote the osteogenic differentiation and inhibit the adipogenic differentiation of ASCs.
微小RNA在干细胞的增殖和分化中发挥着重要作用，但微小RNA调节脂肪干细胞（ASC）成骨或成脂分化的机制仍知之甚少。在本研究中，结果表明微小RNA-196a的上调能够促进ASC的成骨分化，而微小RNA-196a的下调则诱导成脂分化。进一步研究表明，微小RNA-196a可调节Wnt信号通路，以影响ASC的成骨或成脂分化，添加Wnt激动剂1能够逆转由微小RNA-196a缺乏引起的ASC成骨分化下调，而用XAV939抑制Wnt信号通路则促进了ASC的成脂分化。综上所述，微小RNA-196a可能通过调节Wnt信号通路来促进ASC的成骨分化并抑制其成脂分化。
