Wang J Y, Nishiyama K, Araki K, Kitamura D, Watanabe T
Department of Molecular Immunology, Kyushu University, Fukuoka, Japan.
Nucleic Acids Res. 1987 Dec 23;15(24):10105-16. doi: 10.1093/nar/15.24.10105.
The highly conserved octamer sequence ATGCAAAT or its inverse complement found in all human and murine immunoglobulin gene promoters has been demonstrated to be necessary in the lymphoid-specific transcription by deletion analysis. Trans-acting factors that interact with the octamer motif are thought to be involved in this tissue-specific expression. Using a gel mobility shift assay, we have identified both lymphoid-specific and ubiquitous nuclear factors that interact with a human gamma 1 heavy chain gene promoter region containing the octamer motif, consistent with the results obtained with murine heavy or light chain promoter regions. We have purified an octamer binding protein from human B cells by sequence-specific DNA affinity chromatography. Renaturation of gel-purified protein allowed the identification of a polypeptide with a molecular weight of 74 kilodaltons (kD) that is capable of recognizing and binding to the octamer motif. This 74 kD protein seems to be also present in T-cells and non-lymphoid cells. The possible function of the factor is discussed.
在所有人类和小鼠免疫球蛋白基因启动子中发现的高度保守的八聚体序列ATGCAAAT或其反向互补序列，通过缺失分析已证明在淋巴特异性转录中是必需的。与八聚体基序相互作用的反式作用因子被认为参与了这种组织特异性表达。使用凝胶迁移率变动分析，我们已经鉴定出与包含八聚体基序的人类γ1重链基因启动子区域相互作用的淋巴特异性和普遍存在的核因子，这与用小鼠重链或轻链启动子区域获得的结果一致。我们通过序列特异性DNA亲和色谱从人B细胞中纯化了一种八聚体结合蛋白。凝胶纯化蛋白的复性使得能够鉴定出一种分子量为74千道尔顿（kD）的多肽，其能够识别并结合八聚体基序。这种74 kD蛋白似乎也存在于T细胞和非淋巴细胞中。讨论了该因子的可能功能。
