Ericsson Aaron C, Busi Susheel B, Amos-Landgraf James M
Department of Veterinary Pathology, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA.
Methods Mol Biol. 2019;2018:195-212. doi: 10.1007/978-1-4939-9581-3_9.
It is becoming increasingly apparent that microbiota have measurable effects on numerous phenotypes in laboratory animals. This "second genome" has often been disregarded or ignored due to its commensal nonpathogenic nature, and the difficulty, expense, and analysis of sequence. Recent advances in sequencing methods and analyses of large datasets have made characterization of microbiota populations routine and have uncovered previously unknown relationships of microbial communities and host biological systems. The largest and most diverse microbial community in the laboratory rat is in the gut, and has been shown to affect the physiology of the whole animal, and genetic disease penetrance. We present here a cost-effective method for the characterization of the rat fecal microbiota through multiplexed 16S ribosomal sequencing and freely available software.
越来越明显的是，微生物群对实验动物的众多表型具有可测量的影响。由于其共生非致病性质以及序列分析的难度、成本和复杂性，这个“第二基因组”常常被忽视或忽略。测序方法和大型数据集分析的最新进展使微生物群群体的表征变得常规化，并揭示了微生物群落与宿主生物系统之间以前未知的关系。实验大鼠中最大且最多样化的微生物群落存在于肠道中，并且已被证明会影响整个动物的生理以及遗传疾病的外显率。我们在此介绍一种通过多重16S核糖体测序和免费软件来表征大鼠粪便微生物群的经济有效方法。
