肠道微生物组学分析。
Profiling the Gastrointestinal Microbiota.
机构信息
Dipartimento di Scienze Mediche e Chirurgiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.
Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.
出版信息
Methods Mol Biol. 2021;2283:83-92. doi: 10.1007/978-1-0716-1302-3_10.
In this chapter, we provide a methodological description of the process to perform gastrointestinal (GIT) microbiota profiling on human stool samples. The process includes: (i) collection of feces, (ii) isolation of DNA from fecal community bacteria, (iii) selection of both 16S rDNA sequencing target and next-generation sequencing platform, and (iv) analysis and interpretation of sequence data. The process culminates into a comprehensive report on the GIT microbiota composition and structure that may translate into clinically actionable results.
在本章中，我们提供了一种对人类粪便样本进行胃肠道（GIT）微生物组分析的方法描述。该过程包括：（i）粪便采集，（ii）从粪便菌群中分离 DNA，（iii）选择 16S rDNA 测序目标和下一代测序平台，以及（iv）对序列数据进行分析和解释。该过程最终生成一份关于 GIT 微生物群落组成和结构的综合报告，可能转化为临床可操作的结果。
Zotero 插件