Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu/Huai'an Key Laboratory for Agricultural Biotechnology, Huai'an 223001, China.
Soybean research institution, National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Int J Mol Sci. 2019 Jun 21;20(12):3041. doi: 10.3390/ijms20123041.
Soybean is a globally important legume crop that provides a primary source of high-quality vegetable protein and oil. Seed protein and oil content are two valuable quality traits controlled by multiple genes in soybean. In this study, the restricted two-stage multi-locus genome-wide association analysis (RTM-GWAS) procedure was performed to dissect the genetic architecture of seed protein and oil content in a diverse panel of 279 soybean accessions from the Yangtze and Huaihe River Valleys in China. We identified 26 quantitative trait loci (QTLs) for seed protein content and 23 for seed oil content, including five associated with both traits. Among these, 39 QTLs corresponded to previously reported QTLs, whereas 10 loci were novel. As reported previously, the QTL on chromosome 20 was associated with both seed protein and oil content. This QTL exhibited opposing effects on these traits and contributed the most to phenotype variation. From the detected QTLs, 55 and 51 candidate genes were identified for seed protein and oil content, respectively. Among these genes, eight may be promising candidate genes for improving soybean nutritional quality. These results will facilitate marker-assisted selective breeding for soybean protein and oil content traits.
大豆是一种全球重要的豆类作物，为高质量植物蛋白和油的主要来源。种子蛋白和油含量是由大豆中多个基因控制的两个有价值的品质性状。本研究中，采用受限两阶段多基因座全基因组关联分析（RTM-GWAS）程序，对来自中国长江和淮河流域的 279 份大豆种质资源的种子蛋白和油含量的遗传结构进行了剖析。我们鉴定到了 26 个与种子蛋白含量相关的数量性状位点（QTL）和 23 个与种子油含量相关的 QTL，其中包括 5 个与这两个性状都相关的 QTL。其中，有 39 个 QTL 与先前报道的 QTL 相对应，而有 10 个位点是新的。如前所述，20 号染色体上的 QTL 与种子蛋白和油含量都有关。该 QTL 对这两个性状表现出相反的作用，对表型变异的贡献最大。从检测到的 QTL 中，分别鉴定到了 55 个和 51 个与种子蛋白和油含量相关的候选基因。在这些基因中，有 8 个可能是提高大豆营养品质的有前途的候选基因。这些结果将有助于大豆蛋白和油含量性状的标记辅助选择育种。
