Faculty of Chemical and Metallurgical Engineering, Food Engineering Department, Yıldız Technical University, Istanbul, Turkey.
Faculty of Pharmacy, Department of Pharmaceutical Technology, Istanbul University, Istanbul, Turkey.
Biotechnol Prog. 2019 Nov;35(6):e2876. doi: 10.1002/btpr.2876. Epub 2019 Jul 18.
Reactive oxygen species can bind protein, DNA, lipids, and carbohydrates and thus cause an oxidation reaction that induces various syndromes such as cardiovascular diseases, degenerative disease, and cancer types in the human body. Bioactive compounds, such as PUFA, EPA, DHA, and carotenoids in algae, have a chain ring and protect the tissue from chemical damage and reverse the symptoms of some diseases. Algal bioactives also have various biological properties such as anticoagulants, antiviral, antiangiogenic, antitumor, anti-inflammatory, antioxidant, antiproliferative, and immune modulation properties. This study aimed to show in vitro cytotoxic activity effect of Chlorella protothecoides and Nannochloropsis oculata microalgal extracts loaded nano-microparticles on A-172 (Homo sapiens brain glioblastoma) and HCT-116 (H. sapiens colon colorectal carcinoma) cell lines because of the increasing importance of algal biotechnology. MTT viability tests were performed on HUVEC, A172, and HCT 116 cells with particles obtained at optimum process parameters. The cell viability rates of encapsulated particles were also compared with pure algae extracts. Microalgal extracts loaded nano-micro particles showed very promising results for cytotoxic effect on cancer cells.
活性氧可以与蛋白质、DNA、脂质和碳水化合物结合，从而引起氧化反应，导致心血管疾病、退行性疾病和癌症等各种综合征在人体中发生。藻类中的多不饱和脂肪酸（PUFA）、二十碳五烯酸（EPA）、二十二碳六烯酸（DHA）和类胡萝卜素等生物活性化合物具有链环结构，可保护组织免受化学损伤，并逆转某些疾病的症状。藻类生物活性物质还具有各种生物特性，如抗凝剂、抗病毒、抗血管生成、抗肿瘤、抗炎、抗氧化、抗增殖和免疫调节特性。本研究旨在展示 Chlorella protothecoides 和 Nannochloropsis oculata 微藻提取物负载纳米-微粒在 A-172（智人脑神经胶质细胞瘤）和 HCT-116（智人结肠直肠结肠癌）细胞系中的体外细胞毒性作用，因为藻类生物技术的重要性日益增加。在最佳工艺参数下，用得到的颗粒对 HUVEC、A172 和 HCT116 细胞进行 MTT 细胞活力试验。封装颗粒的细胞活力率也与纯藻类提取物进行了比较。负载微藻提取物的纳米微粒在癌细胞的细胞毒性作用方面显示出非常有前景的结果。
