Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yıldız Technical University, Esenler-Istanbul, Turkey.
Artif Cells Nanomed Biotechnol. 2021 Dec;49(1):381-389. doi: 10.1080/21691401.2021.1906263.
Many of the bioactive substances used in pharmaceutical industry are easily affected by temperature, light and oxygen, and are easily degraded during storage and processing, and exhibit poor adsorption properties during digestion, which limits their direct use. Microalgae are rich in oils which have antimicrobial properties and antioxidants that attract attention in both food and pharmaceutical sectors in recent years. Studies to encapsulate bioactive compound-rich microalgae oils with nanotechnological approaches to improve the physical and chemical stability are relatively new, and it is promising to apply these approaches for pharmaceutical purposes. In this study, cytotoxic effects of oil extracts of and and their oil-loaded nanoparticles on L929 cell line, PC-3 prostate cell line, SHSY-5Y neuroblastoma cell line and AGS gastric adenocarcinoma cell line were investigated. The obtained extracts were found to have no cytotoxic effect on L929 cells. However, they showed cytotoxic effect on cancer cells. As for the nanoparticles; a gradual release was determined and the stability of the nanoparticle structure was shown. In the light of obtained findings, it was considered that nanoparticles produced with oil extracts of microalgae which have bioactive substances, have potential to be evaluated especially in pharmaceutical and cosmetic fields.
许多用于制药行业的生物活性物质很容易受到温度、光照和氧气的影响，在储存和加工过程中容易降解，并且在消化过程中表现出较差的吸附性能，这限制了它们的直接使用。近年来，富含具有抗菌和抗氧化特性的油脂的微藻引起了食品和制药领域的关注。用纳米技术方法将富含生物活性化合物的微藻油进行包封以提高物理和化学稳定性的研究相对较新，并且有望将这些方法应用于制药目的。在这项研究中，研究了 和 的油提取物及其载油纳米粒子对 L929 细胞系、PC-3 前列腺癌细胞系、SHSY-5Y 神经母细胞瘤细胞系和 AGS 胃腺癌细胞系的细胞毒性作用。结果表明，所得提取物对 L929 细胞没有细胞毒性作用，但对癌细胞有细胞毒性作用。对于纳米粒子，确定了其逐步释放，并显示了纳米粒子结构的稳定性。根据获得的结果，考虑到用具有生物活性物质的微藻油制成的纳米粒子具有潜力，特别是在制药和化妆品领域进行评估。
