Bioengineering Department, Yıldız Technical University, 34220, Istanbul, Turkey.
Protein J. 2021 Jun;40(3):377-387. doi: 10.1007/s10930-021-09975-8. Epub 2021 Mar 23.
Microalgal biotechnology has increased rapidly owing to have high value bioactive compounds and numerous consumer products that can be utilized from microalgae. With the development of novel cultivation and processing methods, microalgal biotechnology can meet the high demands of food, energy and pharmaceutical industries. In this context, especially for food and pharmaceutical applications, encapsulation of microalgal bioactive compounds is carried out to protect the compound from oxidation and degradation. In this study, a microalgal production process was carried out and microalgal oil loaded bovine serum albumin (BSA) nanoparticle production using glucose as cross-linking agent was investigated. The influences of different process parameters such as initial BSA concentration, glucose concentration and desolvation temperature on the size of BSA nanoparticles were investigated to achieve very small size nanoparticles. Furthermore, data obtained from the experiments were assessed statistically to model the process. It was found that the obtained nanoparticles showed spherical shape with the mean particle size of around 200-300 nm with zeta potential of about - 23 mV. Also, stability test showed that, there was not any change in particle size for one month storage and nanoparticle structure enhance the protection of microalgae oil from oxidation. At last, antibacterial effect of nanoparticles was presented against E. coli ATCC 8739 and L. monocytogenes ATCC 13932. In here, we demonstrated a microalgal bioprocess which consists of microalgae production to obtain microalgal oil riched in bioactive and, encapsulation of microalgal oil to protect it from environmental conditions.
微藻生物技术由于具有高价值的生物活性化合物和许多可从微藻中利用的消费品而迅速发展。随着新型培养和加工方法的发展，微藻生物技术可以满足食品、能源和制药工业的高需求。在这种情况下，特别是对于食品和制药应用，微藻生物活性化合物的包封是为了保护化合物免受氧化和降解。在本研究中，进行了微藻生产工艺，并研究了使用葡萄糖作为交联剂将微藻油负载到牛血清白蛋白（BSA）纳米颗粒中的生产。研究了不同工艺参数（如初始 BSA 浓度、葡萄糖浓度和去溶剂化温度）对 BSA 纳米颗粒尺寸的影响，以实现非常小的纳米颗粒尺寸。此外，对实验数据进行了统计评估，以对该工艺进行建模。结果发现，所得到的纳米颗粒呈球形，平均粒径约为 200-300nm，zeta 电位约为-23mV。此外，稳定性测试表明，在一个月的储存期内，粒径没有任何变化，纳米颗粒结构增强了对微藻油氧化的保护。最后，展示了纳米颗粒对 E.coli ATCC 8739 和 L.monocytogenes ATCC 13932 的抗菌作用。在这里，我们展示了一个微藻生物工艺，包括微藻生产以获得富含生物活性的微藻油，以及微藻油的包封以保护其免受环境条件的影响。
