Andrade Mariza Araújo Morais de Moura, Alves Juliana Paula Martins, Galvão Iolly Tábata Oliveira Marques, Cavalcanti Camila Muniz, Silva Maria Raquel Lopes, Conde Alfredo José Herrera, Bezerra Alessandra Façanha, Fernandes César Carneiro Linhares, Teixeira Dárcio Italo Alves, Rondina Davide
Faculdade de Medicina Veterinária, Universidade Estadual do Ceará, Fortaleza, CE, Brasil.
Anim Reprod. 2022 Jun 1;19(2):e20200025. doi: 10.1590/1984-3143-AR2020-0025. eCollection 2022.
This study examined the effect of glycerin supply strategies in different short-term protocols on follicular dynamics and ovulatory rate in Morada Nova sheep. Eighteen Morada Nova ewes with body condition > 2.9 had their estrus and follicular waves synchronized using three injections of prostaglandin analogue at seven-day intervals. All animals received the same diet during 21 days, which consisted of a total mixed ration (TMR) based on chopped elephant grass and concentrate twice daily. In the control group (n=9), ewes were fed the TMR diet. In the other four groups, ewes received 150 mL of glycerol daily, supplied as an oral drench or mixed in the TMR during three or seven days prior to the application of the third PGF2 alfa analogue. These groups were named as follows: Drench3d (n=10), Drench7d (n=8), TMR3d (n=9) and TMR7d (n=9). Follicle dynamics were monitored by ultrasonography, and plasma glucose and glutathione peroxidase levels were measured at the third prostaglandin administration. Six days after the final PGF2 alfa analogue dose, ovulatory rate was measured by laparoscopy. Glucose was higher (P< 0.001) in the glycerin-treated groups than in control group (83.7 ± 1.7 vs. 68.4 ± 4.5 mg. dL; P < 0.001). Ewes in the TMR3d, Drench7d and TMR7d groups had a greater (P < 0.001) number of large follicles (≥ 3 < 5 mm), and the presence of follicles larger than 5 mm was observed. In the same groups, at the third PGF2 alfa analogue dose, a greater (P < 0.001) number of growing follicles (> 3 mm) and a larger size of the largest follicle (P < 0.001) were also recorded. Ovulation rate was 30% higher in the groups that received glycerin for seven days (1.6 ± 0.1 53 vs. 1.1 ± 0.1; P < 0.05), and they also exhibited a 38% reduction in glutathione peroxidase. Thus, the use of glycerin in Morada Nova sheep as a source of energy in short-term supplementation for increase ovulation rate is an efficient strategy when provided for seven days, either orally or in the feed.
本研究考察了不同短期方案中的甘油供应策略对摩拉达诺瓦绵羊卵泡动态和排卵率的影响。18只体况评分>2.9的摩拉达诺瓦母羊，每隔7天注射三次前列腺素类似物，使其发情和卵泡波同步。在21天内，所有动物都接受相同的日粮，即基于切碎象草和精料的全混合日粮（TMR），每天投喂两次。对照组（n = 9）的母羊饲喂TMR日粮。在其他四组中，母羊在第三次注射PGF2α类似物前3天或7天，每天接受150 mL甘油，通过口服灌服或混入TMR中供给。这些组命名如下：灌服3天组（n = 10）、灌服7天组（n = 8）、TMR 3天组（n = 9）和TMR 7天组（n = 9）。通过超声监测卵泡动态，并在第三次注射前列腺素时测定血浆葡萄糖和谷胱甘肽过氧化物酶水平。在最后一次注射PGF2α类似物6天后，通过腹腔镜检查测定排卵率。甘油处理组的葡萄糖水平高于对照组（P<0.001）（83.7±1.7 vs. 68.4±4.5 mg.dL；P<0.001）。TMR 3天组、灌服7天组和TMR 7天组的大卵泡（≥3<5 mm）数量更多（P<0.001），并且观察到有大于五毫米的卵泡。在同一组中，在第三次注射PGF2α类似物时，生长卵泡（>3 mm）数量更多（P<0.001），最大卵泡的尺寸更大（P<0.001）。接受甘油7天的组排卵率高30%（1.6±0.153 vs. 1.1±0.1；P<0.05），并且它们的谷胱甘肽过氧化物酶也降低了38%。因此，在摩拉达诺瓦绵羊中，将甘油作为短期补充能量的来源以提高排卵率，无论是口服还是添加到饲料中，连续供应7天都是一种有效的策略。
