Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China.
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China.
Can J Microbiol. 2019 Sep;65(9):642-652. doi: 10.1139/cjm-2018-0588. Epub 2019 Jun 26.
The phyllosphere, which supports a large number of microorganisms, represents the interface between the aboveground parts of plants and air. In this study, four clone libraries were constructed from the phyllosphere of (L), (P), (X), and (Y). Clones related to (L, 12.1%; X, 15.6%; Y, 62.5%; P 70.8%), (X, 2.1%; P, 15.1%; L, 63.7%), (X, 68.8%), (L, 3.3%; P, 7.6%), and (P, 0.9%; L, 4.4%, X; 5.2%, Y; 19.6%) were present at high percentages, highlighting their critical role in contributing nitrogen to the phyllosphere ecosystem. The 16S rDNA sequence analysis suggested that phyllosphere-associated bacteria were affiliated with a wide range of taxa, encompassing members from , , , , , , , , , and -. Additionally, the abundance of the gene and 16S rDNA was assessed with quantitative PCR. The number of copies of and 16S rDNA ranged from 1.14 × 10 to 1.49 × 10 and from 3.72 × 10 to 7.02 × 10 copies/g fresh leaf sample, respectively. In conclusion, our work sheds light on the microbial communities of the phyllosphere that are important for plant growth. Moreover, we observed a unique composition of nitrogen-fixing bacteria in each phyllosphere sample, suggesting the existence of specific interactions between these functional microorganism and plants, which may provide information or be a reference for the development of bacterial fertilizers.
叶片是植物地上部分与空气的接触面，其上生活着大量微生物，构成了叶际微生物区系。本研究构建了 (L)、 (P)、 (X)和 (Y)叶际 4 个克隆文库。克隆文库中与 (L、12.1%；X、15.6%；Y、62.5%；P 70.8%)、 (X、2.1%；P、15.1%；L、63.7%)、 (X、68.8%)、 (L、3.3%；P、7.6%)和 (P、0.9%；L、4.4%；X、5.2%；Y、19.6%)关系密切的序列相对丰度较高，表明它们在向叶际生态系统提供氮素方面发挥着重要作用。16S rDNA 序列分析表明，叶际相关细菌与广泛的分类群相关，包括 、 、 、 、 、 、 、 和 -。此外，还采用定量 PCR 评估了 基因和 16S rDNA 的丰度。和 16S rDNA 的拷贝数范围分别为 1.14×10 到 1.49×10 和 3.72×10 到 7.02×10 拷贝/g 新鲜叶片样本。总之，本研究揭示了对植物生长很重要的叶际微生物群落，而且我们在每个叶际样本中都观察到了固氮细菌的独特组成，这表明这些功能微生物与植物之间存在特定的相互作用，这可能为细菌肥料的开发提供信息或参考。
