Park Sooyeon, Park Ji-Min, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon 16419, South Korea.
Int J Syst Evol Microbiol. 2019 Sep;69(9):2750-2754. doi: 10.1099/ijsem.0.003553.
A Gram-stain-negative, aerobic, non-motile and rod-shaped or filamentous bacterial strain, designated SHSM-M15, was isolated from a salt marsh at Siheung in Republic of Korea and identified by polyphasic taxonomic study. Strain SHSM-M15 grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain SHSM-M15 clusters with the type strain of , showing 16S rRNA gene sequence similarity of 97.8 %. Strain SHSM-M15 had 16S rRNA gene sequence similarities of less than 93.7 % with the type strains of other recognised species. Strain SHSM-M15 contained MK-7 as the predominant menaquinone and iso-C, iso-C 3-OH and anteiso-C as the major fatty acids. The major polar lipid detected in strain SHSM-M15 was phosphatidylethanolamine. The DNA G+C content of strain SHSM-M15 from genomic sequence was 36.6 %. Mean DNA-DNA relatedness value between strain SHSM-M15 and the type strain of was 18 % and the average nucleotide identity value between strain SHSM-M15 and the type strain of was 87.98 %. The phylogenetic and genetic data and differential phenotypic properties indicated that strain SHSM-M15 is separated from . On the basis of the polyphasic data presented, strain SHSM-M15 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SHSM-M15 (=KACC 19862=NBRC 113749).
从韩国始兴的盐沼中分离出一株革兰氏阴性、需氧、无运动性、杆状或丝状的细菌菌株，命名为SHSM-M15，并通过多相分类研究进行了鉴定。菌株SHSM-M15在25°C、pH 7.0 - 8.0以及1.0 - 2.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的系统发育树表明，菌株SHSM-M15与[具体属名]的模式菌株聚类，16S rRNA基因序列相似性为97.8%。菌株SHSM-M15与其他已确认物种的模式菌株的16S rRNA基因序列相似性低于93.7%。菌株SHSM-M15以MK-7作为主要甲基萘醌，以异-C、异-C₃-OH和anteiso-C作为主要脂肪酸。在菌株SHSM-M15中检测到的主要极性脂是磷脂酰乙醇胺。来自基因组序列的菌株SHSM-M15的DNA G+C含量为36.6%。菌株SHSM-Ml5与[具体属名]模式菌株之间的平均DNA-DNA相关性值为18%，菌株SHSM-M15与[具体属名]模式菌株之间的平均核苷酸同一性值为87.98%。系统发育和遗传数据以及不同的表型特征表明，菌株SHSM-M15与[具体属名]分离。基于所呈现的多相数据，菌株SHSM-M15被认为代表[具体属名]属的一个新物种，为此提出新物种名称[具体新物种名] sp. nov.。模式菌株为SHSM-M15（=KACC 19862=NBRC 113749）。
