Frontiers Science Center for Deep Ocean Multispheres and Earth System, and College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003, People's Republic of China.
Laboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao, 266237, People's Republic of China.
Antonie Van Leeuwenhoek. 2024 Sep 21;118(1):8. doi: 10.1007/s10482-024-02022-3.
A novel bacterial strain, designated DW002, was isolated from the sea ice of Cape Evans, McMurdo Sound, Antarctica. Cells of the strain were Gram-negative, obligate anaerobic, motile, non-flagellated, and short rod-shaped. The strain DW002 grew at 4-32 ℃ (optimum at 22-28 ℃) and thrived best at pH 7.0, NaCl concentration of 2.5% (w/v). The predominant isoprenoid quinone of strain DW002 was menaquinone-7 (MK-7). The major fatty acids (> 10%) of DW002 were iso-C, anteiso-C and iso-Cω9c. The predominant polar lipids of strain DW002 contained two phosphatidylethanolamines, one unidentified glycolipid, one unidentified aminolipid and four unidentified lipids. The DNA G + C content of the strain DW002 was 34.8%. Strain DW002 encoded 237 carbohydrate-active enzymes. The strain DW002 had genes associated with dissimilatory nitrate reduction and assimilatory sulfate reduction metabolic pathways. Based on distinct physiological, chemotaxonomic, genome analysis and phylogenetic differences compared to other members of the phylogenetically related genera in the family Marinifilaceae, strain DW002 is proposed to represent a novel genus within the family. Therefore, the name Paralabilibaculum antarcticum gen. nov., sp. nov. is proposed. The type strain is DW002 (=KCTC 25274=MCCC 1K06067).
从南极洲麦克默多海峡埃文斯角的海冰中分离到一株新的细菌菌株，命名为 DW002 菌株。该菌株的细胞呈革兰氏阴性、专性厌氧、运动、无鞭毛、短杆状。DW002 株在 4-32℃（最佳生长温度为 22-28℃）下生长，最适 pH 值为 7.0，NaCl 浓度为 2.5%（w/v）。DW002 株的主要类异戊二烯醌为甲萘醌-7（MK-7）。DW002 的主要脂肪酸（>10%）为 iso-C、anteiso-C 和 iso-Cω9c。DW002 株的主要极性脂含有两种磷脂酰乙醇胺、一种未鉴定的糖脂、一种未鉴定的氨基脂和四种未鉴定的脂类。DW002 株的 DNA G+C 含量为 34.8%。DW002 株编码 237 种碳水化合物活性酶。DW002 株具有与异化硝酸盐还原和同化硫酸盐还原代谢途径相关的基因。与 Marinifilaceae 科亲缘关系密切的其他属成员相比，DW002 株在生理、化学分类和基因组分析以及系统发育上存在明显差异，建议将其代表该科的一个新属。因此，提出了 Paralabilibaculum antarcticum 属，新属，sp. nov.。模式菌株为 DW002（=KCTC 25274=MCCC 1K06067）。
