Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup-si, Korea.
Department of Agricultural Convergence Technology, Chonbuk National University, Jeonju-si, Korea.
PLoS One. 2019 Jun 26;14(6):e0218922. doi: 10.1371/journal.pone.0218922. eCollection 2019.
The present study aimed to evaluate the probiotic characteristics of certain microbial strains for potential use as feed additives. Three bacterial strains and a yeast previously isolated from different environments were investigated. The strains were subjected to molecular identification and established as Lactobacillus paracasei CP133, Lactobacillus plantarum CP134, Bacillus subtilis CP350 and Saccharomyces cerevisiae CP605. Lactobacillus sp. CP133 and CP134 exhibited antibiosis, antibiotic activity, and relative odor reduction ability. Bacillus subtilis CP350 was thermotolerant, reduced hydrogen sulfide gas and showed significant proteolytic activity, whereas Saccharomyces cerevisiae CP605 exhibited high acid and bile salt tolerance. In general, the isolates in this study demonstrated improved functional characteristics, particularly acid and bile tolerance and relative cell adhesion to HT-29 monolayer cell line. Results in this work provides multifunctional probiotic characteristics of the strains for potential development of probiotics and cleaning of the environment.
本研究旨在评估某些微生物菌株的益生菌特性，以期将其用作饲料添加剂。研究了从不同环境中分离出的三种细菌菌株和一种酵母。对菌株进行了分子鉴定，确定为副干酪乳杆菌 CP133、植物乳杆菌 CP134、枯草芽孢杆菌 CP350 和酿酒酵母 CP605。乳杆菌 CP133 和 CP134 表现出抑菌、抗生素活性和相对气味减少能力。枯草芽孢杆菌 CP350 具有耐热性，可减少硫化氢气体并表现出显著的蛋白水解活性，而酿酒酵母 CP605 则具有较高的耐酸和耐胆盐能力。总的来说，本研究中的分离株表现出改善的功能特性，特别是对酸和胆盐的耐受性以及相对细胞对 HT-29 单层细胞系的黏附能力。本工作的结果为这些菌株的多功能益生菌特性提供了依据，有望开发益生菌和清洁环境。
