Zhang Ning, Ma Xiaomin, Li Ru, Xue Yihua, Sun Yeshuo, Nie Shanshan, Zhang Lugang
1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100 Shaanxi China.
State Key Laboratory of Vegetable Germplasm Innovation, Tianjin, 300384 China.
3 Biotech. 2019 Jul;9(7):274. doi: 10.1007/s13205-019-1813-6. Epub 2019 Jun 19.
To identify genes associated with carotenoid accumulation in petals of Chinese cabbage, the composition and content of carotenoids were analyzed, and comparative transcriptome sequencing was performed between the yellow flower line, 92S105, and the orange flower line, 94C9. High-performance liquid chromatography (HPLC) revealed that petals of 92S105 were high in violaxanthin as well as lutein, whereas petals of 94C9 showed considerable levels of lutein and β-carotene. Transcriptome analysis showed that 3534 and 3833 genes were up- and down-regulated in 94C9, respectively. Among these differentially expressed genes (DEGs), many related to carotenoid accumulation were identified, including 12 carotenoid biosynthesis pathway genes, 4 transcription factor genes, and 1028 specifically expressed genes. - (), , (), and MYB transcription factor gene () were down-regulated in petals of 94C9 when compared with petals of 92S105, which caused β-carotene accumulation and may lead to orange petal color in 94C9. Expression levels of 20 DEGs were verified by qPCR and the results were highly consistent with those of transcriptome sequencing. Moreover, Gene Ontology (GO) enrichment analysis revealed that membrane, binding, and metabolic processes were the most significantly enriched GO terms in cellular component, molecular function, and biological process ontologies, respectively. In conclusion, our study analyzed the differences in composition and content of carotenoids between 92S105 and 94C9 and identified potential candidate genes related to carotenoid accumulation in petals, thereby creating a solid foundation for future studies on the mechanism regulating carotenoid accumulation in petals of Chinese cabbage.
为了鉴定与大白菜花瓣中类胡萝卜素积累相关的基因，分析了类胡萝卜素的组成和含量，并对黄花品系92S105和橙花品系94C9进行了比较转录组测序。高效液相色谱（HPLC）分析表明，92S105花瓣中紫黄质和叶黄素含量较高，而94C9花瓣中叶黄素和β-胡萝卜素含量较高。转录组分析显示，94C9中分别有3534个和3833个基因上调和下调。在这些差异表达基因（DEG）中，鉴定出许多与类胡萝卜素积累相关的基因，包括12个类胡萝卜素生物合成途径基因、4个转录因子基因和1028个特异性表达基因。与92S105花瓣相比，94C9花瓣中的（）、（）、（）和MYB转录因子基因（）下调，这导致了β-胡萝卜素的积累，并可能导致94C9花瓣呈现橙色。通过qPCR验证了20个DEG的表达水平，结果与转录组测序结果高度一致。此外，基因本体（GO）富集分析表明，膜、结合和代谢过程分别是细胞组分、分子功能和生物过程本体中最显著富集的GO术语。总之，本研究分析了92S105和94C9之间类胡萝卜素组成和含量的差异，鉴定出与花瓣中类胡萝卜素积累相关的潜在候选基因，从而为今后大白菜花瓣中类胡萝卜素积累调控机制的研究奠定了坚实基础。
