Mansour M M, Dunn M A, Salah L A, Woody J N
Biochemistry Department, U.S. Naval Medical Research Unit, Cairo, Egypt.
Am J Trop Med Hyg. 1988 Jan;38(1):111-7. doi: 10.4269/ajtmh.1988.38.111.
Primary cell cultures from the livers of mice infected with Schistosoma mansoni were prepared and cells with the appearance of fibroblasts by light microscopy were isolated. Collagen synthesis was estimated by measuring incorporation of 14C-proline into collagenase-sensitive proteins for both culture media and cell layers. Coculture of splenic T cells from infected mice with these hepatic fibroblasts caused greater selective and specific reduction in collagen production than did coculture using spleen cells from normal mice. There was a parallel inhibition in collagen within the cell layer which indicates that the marked decrease in collagen production was due to inhibition of synthesis and not related to changes in solubility or secretion. Primary culture of mouse skin fibroblasts showed similar responses to coculture but an established fibroblast line, 3T3, was unresponsive. Inflammatory cells appear to influence hepatic fibroblasts isolated under our experimental condition in several ways, such as opposite effects on collagen synthesis and cell proliferation.
制备感染曼氏血吸虫的小鼠肝脏的原代细胞培养物，并通过光学显微镜分离出具有成纤维细胞外观的细胞。通过测量14C-脯氨酸掺入培养基和细胞层中对胶原酶敏感的蛋白质来估计胶原合成。与正常小鼠脾脏细胞共培养相比，感染小鼠脾脏T细胞与这些肝成纤维细胞共培养导致胶原产生的选择性和特异性降低更大。细胞层内胶原存在平行抑制，这表明胶原产生的显著减少是由于合成抑制，而与溶解度或分泌的变化无关。小鼠皮肤成纤维细胞的原代培养对共培养表现出类似反应，但已建立的成纤维细胞系3T3无反应。炎症细胞似乎在我们的实验条件下以多种方式影响分离出的肝成纤维细胞，例如对胶原合成和细胞增殖的相反作用。
