Federal University of Alagoas, Institute of Pharmaceutical Sciences, Maceió, AL, Brazil.
Federal University of Alagoas, Institute of Biology and Health Science, Maceió, AL, Brazil.
J Ethnopharmacol. 2019 Oct 5;242:112037. doi: 10.1016/j.jep.2019.112037. Epub 2019 Jun 24.
The leaves of Alpinia zerumbet is used in folk medicine in Brazil to treat hypertension. However, the cardioprotective effect of this plant has not been studied yet.
To evaluate the cardioprotective effects of the hydroalcoholic extract of the leaves of Alpinia zerumbet (AZE) against isoproterenol (ISO)-induced myocardial infarction in rats.
Rats were pretreated orally with AZE (300 mg/kg) for 30 days prior to ISO-induced myocardial infarction. The rats were sacrificed and hearts were collected and homogenized for biochemical analysis. At the end of the experiment, cardiac marker enzyme levels, histological and morphometric parameters, and hemodynamic measurements were assessed. Phytochemical compounds were verified by gas chromatography-mass spectrometry (GC-MS).
Rats administered with ISO showed a significant increase in cardiac marker enzymes, i.e., in creatine kinase-NAC (CK-NAC) and CK-MB. Triphenyltetrazolium chloride (TTC) staining exhibited an increase in infarct areas. In the animals treated with ISO induced a significant increase in heart rate. Pretreatment with AZE significantly inhibited these effects of ISO. Moreover, biochemical findings were supported by histopathological observations. The GC-MS analyses of AZE identified volatile oils, kavalactones, and phytosterols.
Haemodynamic, biochemical alteration and histopathological results suggest a cardioprotective protective effect of oral administration of AZE in isoproterenol induced cardiotoxicity.
巴西民间医学中使用益智的叶子来治疗高血压。然而，这种植物的心脏保护作用尚未得到研究。
评估益智叶水醇提取物（AZE）对异丙肾上腺素（ISO）诱导的大鼠心肌梗死的心脏保护作用。
ISO 诱导心肌梗死前，大鼠预先经口给予 AZE（300mg/kg）30 天。处死大鼠，收集心脏并匀浆进行生化分析。实验结束时，评估心脏标志物酶水平、组织学和形态计量学参数以及血液动力学测量。通过气相色谱-质谱联用（GC-MS）验证植物化学成分。
给予 ISO 的大鼠心脏标志物酶，即肌酸激酶-NAC（CK-NAC）和 CK-MB 显著增加。三苯基四氮唑氯化物（TTC）染色显示梗死面积增加。ISO 诱导的动物心率显著增加。AZE 预处理显著抑制了 ISO 的这些作用。此外，生化发现得到组织病理学观察的支持。AZE 的 GC-MS 分析鉴定出挥发油、卡瓦内酯和植物甾醇。
血液动力学、生化改变和组织病理学结果表明，AZE 口服给药对 ISO 诱导的心脏毒性具有心脏保护作用。
