Colegio de Ciencias de la Salud, Escuela de Medicina Veterinaria, Universidad San Francisco de Quito, 17-12-841, Quito, Ecuador.
IRMB CHU Saint Eloi, University of Montpellier, France, 80 rue Augustin Fliche, 34295, Montpellier, cedex 5, France.
BMC Biotechnol. 2019 Jun 28;19(1):42. doi: 10.1186/s12896-019-0534-6.
Artificial Mitochondrial Transfer or Transplant (AMT/T) can be used to reduce the stress and loss of viability of damaged cells. In MitoCeption, a type of AMT/T, the isolated mitochondria and recipient cells are centrifuged together at 4 °C and then co-incubated at 37 °C in normal culture conditions, inducing the transfer. Ultraviolet radiation (UVR) can affect mitochondria and other cell structures, resulting in tissue stress, aging, and immunosuppression. AMT/T could be used to repair UVR cellular and mitochondrial damage. We studied if a mitochondrial mix from different donors (Primary Allogeneic Mitochondrial Mix, PAMM) can repair UVR damage and promote cell survival.
Using a simplified adaption of the MitoCeption protocol, we used peripheral blood mononuclear cells (PBMCs) as the recipient cell model of the PAMM in order to determine if this protocol could repair UVR damage. Our results showed that when PBMCs are exposed to UVR, there is a decrease in metabolic activity, mitochondrial mass, and mtDNA sequence stability as well as an increase in p53 expression and the percentage of dead cells. When PAMM MitoCeption was used on UVR-damaged cells, it successfully transferred mitochondria from different donors to distinct PBMCs populations and repaired the observed UVR damage.
Our results represent an advancement in the applications of MitoCeption and other AMT/T. We showed that PBMCs could be used as a PAMM source of mitochondria. We also showed that these mitochondria can be transferred in a mix from different donors (PAMM) to UVR-damaged, non-adherent primary cells. Additionally, we decreased the duration of the MitoCeption protocol.
人工线粒体转移或移植（AMT/T）可用于减轻受损细胞的应激和活力损失。在 MitoCeption 中，一种 AMT/T，分离的线粒体和受体细胞在 4°C 下一起离心，然后在正常培养条件下在 37°C 共孵育，诱导转移。紫外线辐射（UVR）会影响线粒体和其他细胞结构，导致组织应激、衰老和免疫抑制。AMT/T 可用于修复 UVR 引起的细胞和线粒体损伤。我们研究了来自不同供体的线粒体混合物（原发性同种异体线粒体混合物，PAMM）是否可以修复 UVR 损伤并促进细胞存活。
使用 MitoCeption 方案的简化适应，我们使用外周血单核细胞（PBMC）作为 PAMM 的受体细胞模型，以确定该方案是否可以修复 UVR 损伤。我们的结果表明，当 PBMC 暴露于 UVR 时，代谢活性、线粒体质量和 mtDNA 序列稳定性下降，p53 表达增加，死亡细胞的百分比增加。当将 PAMM MitoCeption 用于 UVR 损伤的细胞时，它成功地将线粒体从不同的供体转移到不同的 PBMC 群体，并修复了观察到的 UVR 损伤。
我们的结果代表了 MitoCeption 和其他 AMT/T 的应用的进步。我们表明 PBMC 可作为 PAMM 线粒体的来源。我们还表明，这些线粒体可以在来自不同供体（PAMM）的混合物中转移到 UVR 损伤的非贴壁原代细胞。此外，我们缩短了 MitoCeption 方案的持续时间。
