Li Weiping, Tian Shuang, Wang Pengpeng, Zang Yikun, Chen Xin, Yao Yuanqing, Li Weiyang
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, P. R. China.
MyGenostics Inc., Beijing, P. R. China.
J Cancer. 2019 Jun 2;10(12):2783-2787. doi: 10.7150/jca.31450. eCollection 2019.
Cervical cancer is one of the most common malignant tumors in gynecology. Deploying HIVID, a cost-effective technique to detect HPV integration sites, our team had studied the characteristics of HPV integrations in cervical exfoliated cells. Our results indicated that both the sample proportion and the number of HPV integrations gradually increased following the development of cervical lesion. Meanwhile, our data also revealed that there were recurrent genes integrated by HPV in cervical exfoliated cells. Collectively, the HPV integration breakpoints were highly enriched in the intron and promoter regions. Intriguingly, the gene pathway analysis indicated that the HPV-integrated genes were strongly inclined to pathways of metabolism of xenobiotics by cytochrome P450, chemical carcinogenesis and steroid hormone biosynthesis. In conclusion, this study unveiled the HPV integration patterns and the associated recurrent genes in cervical epithelial exfoliated cells. Altogether, our data suggested that the HPV integrations in cervical exfoliated cells might have vital clinical significance, and probably also diagnostic and/or prognostic values in future clinical applications.
宫颈癌是妇科最常见的恶性肿瘤之一。我们的团队采用HIVID（一种检测HPV整合位点的经济高效技术）研究了宫颈脱落细胞中HPV整合的特征。我们的结果表明，随着宫颈病变的发展，HPV整合的样本比例和数量逐渐增加。同时，我们的数据还显示，宫颈脱落细胞中存在HPV反复整合的基因。总体而言，HPV整合断点在基因内含子和启动子区域高度富集。有趣的是，基因通路分析表明，HPV整合的基因强烈倾向于细胞色素P450对外源生物的代谢、化学致癌作用和类固醇激素生物合成等通路。总之，本研究揭示了宫颈上皮脱落细胞中的HPV整合模式及相关反复整合基因。我们的数据表明，宫颈脱落细胞中的HPV整合可能具有重要的临床意义，在未来临床应用中可能还具有诊断和/或预后价值。
