Department of Biology, University of Maryland, College Park, United States.
Department of Biology, West Virginia University, Morgantown, United States.
Elife. 2019 Jul 2;8:e46839. doi: 10.7554/eLife.46839.
Serotonin plays different roles across networks within the same sensory modality. Previously, we used whole-cell electrophysiology in to show that serotonergic neurons innervating the first olfactory relay are inhibited by odorants (Zhang and Gaudry, 2016). Here we show that network-spanning serotonergic neurons segregate information about stimulus features, odor intensity and identity, by using opposing coding schemes in different olfactory neuropil. A pair of serotonergic neurons (the CSDns) innervate the antennal lobe and lateral horn, which are first and second order neuropils. CSDn processes in the antennal lobe are inhibited by odors in an identity independent manner. In the lateral horn, CSDn processes are excited in an odor identity dependent manner. Using functional imaging, modeling, and EM reconstruction, we demonstrate that antennal lobe derived inhibition arises from local GABAergic inputs and acts as a means of gain control on branch-specific inputs that the CSDns receive within the lateral horn.
血清素在同一感觉模态的网络中扮演着不同的角色。此前，我们使用全细胞膜片钳技术在 中显示，支配第一嗅觉中继的血清素能神经元被气味抑制（Zhang 和 Gaudry，2016）。在这里，我们通过在不同的嗅觉神经突中使用相反的编码方案，表明跨网络的血清素能神经元对刺激特征、气味强度和身份的信息进行了分离。一对血清素能神经元（CSDns）支配触角叶和侧角，它们是第一和第二级神经突。CSDn 在触角叶中的处理过程以与身份无关的方式被气味抑制。在侧角中，CSDn 的处理过程以气味身份依赖的方式被兴奋。使用功能成像、建模和 EM 重建，我们证明了来自触角叶的抑制作用来源于局部 GABA 能输入，并作为一种增益控制手段，作用于 CSDns 在侧角中接收的分支特异性输入。
