Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, Modern Separation Science Key Laboratory of Shaanxi Province, College of Chemistry & Materials Science , Northwest University , 710127 , Xi'an , China.
School of Chemical and Biomedical Engineering , Nanyang Technological University , 70 Nanyang Drive , 637457 , Singapore.
Bioconjug Chem. 2019 Aug 21;30(8):2089-2101. doi: 10.1021/acs.bioconjchem.9b00391. Epub 2019 Jul 22.
β-Galactosidase (β-Gal), as a lysosomal hydrolytic enzyme, plays an important physiological role in catalyzing the hydrolysis of glycosidic bonds which convert lactose into galactose. Moreover, upregulation of β-Gal is often correlated with the occurrence of primary ovarian cancers and cell senescence. Thereby, detection of β-Gal activity is relevant to cancer diagnosis. Optical imaging possesses high spatial and temporal resolution, high sensitivity, and real-time imaging capability. These properties are beneficial for the detection of β-Gal in living systems. This Review summarizes the recent progress in development of molecular optical probes for near-infrared fluorescence (NIRF), bioluminescence (BL), chemiluminescence (CL), or photoacoustic (PA) imaging of β-Gal in biological systems. The challenges and opportunities in the probe design for detection of β-Gal are also discussed.
β-半乳糖苷酶（β-Gal）作为溶酶体水解酶，在催化糖苷键水解将乳糖转化为半乳糖的过程中发挥着重要的生理作用。此外，β-Gal 的上调通常与原发性卵巢癌和细胞衰老的发生有关。因此，β-Gal 活性的检测与癌症诊断有关。光学成像是一种具有高时空分辨率、高灵敏度和实时成像能力的方法。这些特性有利于在活体内检测β-Gal。本文综述了近年来用于近红外荧光（NIRF）、生物发光（BL）、化学发光（CL）或光声（PA）成像的用于检测生物体内β-Gal 的分子光学探针的研究进展。文中还讨论了在用于检测β-Gal 的探针设计方面所面临的挑战和机遇。
