Adolphe Merkle Institute , University of Fribourg , Chemin des Verdiers 4 , 1700 Fribourg , Switzerland.
Department of Chemistry , University of Fribourg , Chemin du Musée 9 , 1700 Fribourg , Switzerland.
ACS Nano. 2019 Jul 23;13(7):7759-7770. doi: 10.1021/acsnano.9b01604. Epub 2019 Jul 11.
The long-term fate of biomedically relevant nanoparticles (NPs) at the single cell level after uptake is not fully understood yet. We report that lysosomal exocytosis of NPs is not a mechanism to reduce the particle load. Biopersistent NPs such as nonporous silica and gold remain in cells for a prolonged time. The only reduction of the intracellular NP number is observed cell division, , mitosis. Additionally, NP distribution after cell division is observed to be asymmetrical, likely due to the inhomogeneous location and distribution of the NP-loaded intracellular vesicles in the mother cells. These findings are important for biomedical and hazard studies as the NP load per cell can vary significantly. Furthermore, we highlight the possibility of biopersistent NP accumulation over time within the mononuclear phagocyte system.
生物医学相关纳米颗粒（NPs）在被细胞摄取后，其在单细胞水平的长期命运尚不完全清楚。我们报告称， NPs 通过溶酶体胞吐的方式排出并不是减少颗粒负荷的机制。生物持久性 NPs（如无孔二氧化硅和金）在细胞内会持续存在很长时间。只有在细胞分裂时，才能观察到细胞内 NP 数量的减少，这是有丝分裂的结果。此外，还观察到 NP 在细胞分裂后的分布是不对称的，这可能是由于 NP 负载的细胞内囊泡在母细胞中的位置和分布不均匀所致。这些发现对于生物医学和危害研究很重要，因为每个细胞的 NP 负荷可能会有很大差异。此外，我们还强调了 NP 在单核吞噬细胞系统中随时间积累的可能性。
