Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering , Jiangsu University , Zhenjiang 212013 , P.R. China.
Beijing Research Center for Agricultural Standards and Testing, Risk Assessment Laboratory for Agro-Products (Beijing), Ministry of Agriculture , Beijing Municipal Key Laboratory of Agriculture Environment Monitoring , No. 9 Middle Road of Shu Guang Hua Yuan, Haidian Dist. , Beijing 100097 , P.R. China.
J Agric Food Chem. 2019 Jul 17;67(28):8035-8044. doi: 10.1021/acs.jafc.9b00090. Epub 2019 Jul 8.
The simultaneous detection of multiple mycotoxins is important due to the increased toxic effects of combined mycotoxins in grains. In this research, a combination of modified QuEChERS with ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used for simultaneous detection of 20 mycotoxins in grains. A series of different types of magnetic (FeO) nanoparticles modified with multiwalled carbon nanotubes (FeO-MWCNTs) were designed as modified QuEChERS adsorbents for facile and efficient purification and for target interferences removal in the matrices. When there is an external magnetic field, the proposed modified QuEChERS method uses a shorter pretreatment time compared with the traditional QuEChERS method, which makes it possible to conduct high-throughput analyses. To optimize the QuEChERS process, the extraction solvent and the type and amount of the FeO-MWCNTs were investigated. Under optimal conditions, the method was validated and showed satisfactory linearity ( ≥ 0.9965), good recovery (73.5-112.9%), good precision (1.3-12.7%), and excellent sensitivity (ranging from 0.0021 to 5.4457 ng g), which indicates that this method can be used for detecting multiple mycotoxins in real samples.
由于谷物中组合真菌毒素的毒性增强，因此同时检测多种真菌毒素很重要。本研究采用改良的 QuEChERS 与超高效液相色谱-串联质谱法（UPLC-MS/MS）相结合，用于同时检测谷物中的 20 种真菌毒素。设计了一系列不同类型的磁性（FeO）纳米粒子修饰的多壁碳纳米管（FeO-MWCNTs）作为改良 QuEChERS 吸附剂，用于简便、高效的净化和去除基质中的目标干扰物。在外磁场存在的情况下，与传统的 QuEChERS 方法相比，所提出的改良 QuEChERS 方法采用更短的预处理时间，这使得高通量分析成为可能。为了优化 QuEChERS 工艺，考察了提取溶剂和 FeO-MWCNTs 的类型和用量。在最佳条件下，对该方法进行了验证，结果表明其具有良好的线性关系（≥0.9965）、良好的回收率（73.5-112.9%）、良好的精密度（1.3-12.7%）和优异的灵敏度（范围为 0.0021-5.4457ng g），表明该方法可用于检测实际样品中的多种真菌毒素。
