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通过内切β-半乳糖苷酶从人红细胞糖肽释放的N-乙酰乳糖胺型寡糖的500兆赫¹H核磁共振谱

500 MHz 1H n.m.r. of oligosaccharides of N-acetyl-lactosamine-type released from human erythrocyte glycopeptides by endo-beta-galactosidase.

作者信息

Hounsell E F, Feeney J, Scudder P

机构信息

Applied Immunochemistry Research Group, Clinical Research Centre, Harrow, Middx., U.K.

出版信息

Biochem J. 1988 Feb 15;250(1):9-13. doi: 10.1042/bj2500009.

DOI:10.1042/bj2500009
PMID:3128279
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1148807/
Abstract

500 MHz 1H n.m.r. spectroscopy has been used in structural studies of three linear and five branched oligosaccharides of N-acetyl-lactosamine-type that were released from desialylated blood group O erythrocyte glycopeptides by treatment with the endo-beta-galactosidase of Bacteroides fragilis followed by reduction. The following oligosaccharide alditols were characterized: (formula; see book)

摘要

500兆赫的¹H核磁共振光谱法已用于对三种线性和五种支链的N-乙酰乳糖胺型寡糖进行结构研究，这些寡糖是通过用脆弱拟杆菌的内切β-半乳糖苷酶处理脱唾液酸的O型血红细胞糖肽，然后进行还原反应从其中释放出来的。对以下寡糖醇进行了表征：（分子式；见书籍）

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本文引用的文献

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Primary-structure determination of fourteen neutral oligosaccharides derived from bronchial-mucus glycoproteins of patients suffering from cystic fibrosis, employing 500-MHz 1H-NMR spectroscopy.利用500兆赫的1H核磁共振光谱法对来自囊性纤维化患者支气管黏液糖蛋白的14种中性寡糖进行一级结构测定。
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Structure of branched lactosaminoglycan, the carbohydrate moiety of band 3 isolated from adult human erythrocytes.从成人人类红细胞中分离出的带3糖蛋白的碳水化合物部分——支链乳糖胺聚糖的结构。
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Structure of fetal lactosaminoglycan. The carbohydrate moiety of Band 3 isolated from human umbilical cord erythrocytes.胎儿乳糖胺聚糖的结构。从人脐带红细胞中分离出的带3的碳水化合物部分。
J Biol Chem. 1984 Apr 25;259(8):4782-91.
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A computerized approach to the analysis of oligosaccharide structure by high-resolution proton n.m.r.一种通过高分辨率质子核磁共振对寡糖结构进行分析的计算机化方法
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