Singapore Institute of Manufacturing Technology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Beijing, P. R. China.
Biotechnol Bioeng. 2019 Oct;116(10):2764-2777. doi: 10.1002/bit.27106. Epub 2019 Jul 24.
Bridging the unmet need of efficient point-of-care testing (POCT) in biomedical engineering research and practice with the emerging development in artificial synthetic xeno nucleic acids (XNAs), this review summarized the recent development in paper-based POCT using XNAs as sensing probes. Alongside the signal transducing mode and immobilization methods of XNA probes, a detailed evaluation of probe performance was disclosed. With these new aspects, both researchers in synthetic chemistry / biomedical engineering and physicians in clinical practice could gain new insights in designing, manufacturing and choosing suitable reagents and techniques for POCT.
将人工合成的异源核酸 (XNA) 在生物医学工程研究和实践中的高效即时检测 (POCT) 方面的未满足需求与新兴发展相结合，本综述总结了基于纸张的 XNA 作为传感探针的 POCT 的最新发展。除了 XNA 探针的信号转导模式和固定方法外，还详细评估了探针的性能。通过这些新方面，合成化学/生物医学工程领域的研究人员和临床医生都可以深入了解设计、制造和选择适合 POCT 的试剂和技术。
