Hoffmann M K, Watson J
J Immunol. 1979 Apr;122(4):1371-5.
The biologic activities of helper T cell-replacing factors derived from concanavalin A-stimulated murine T cells (TRF-T) and from lipopolysaccharide-activated macrophages (TFR-M) have been compared. TRF-T stimulates immune responses to heterologous erythrocyte antigens (SRBC and BRBC) in T cell-depleted spleen cultures but not in macrophage-depleted spleen cultures. TRF-M stimulates immune responses in both T cell-depleted and macrophage-depleted spleen cultures. Under conditions where LPS stimulates the release of TRF-M from cultures of activated macrophages, TRF-t has no effect on TFR-M production. Thus. TRF-T does not appear to function by stimulating the release of TRF-M from macrophages. In macrophage-depleted spleen cultures, saturating concentrations of TRF-T and TRF-M when mixed together exhibit striking synergistic effects on the induction of immune responses to erythrocyte antigens. The kinetics of the synergistic effects of TRF-M and TRF-T are consistent with an effect of TRF-M on the production of TRF-T sensitive B cells.
对来自刀豆蛋白A刺激的小鼠T细胞（TRF-T）和脂多糖激活的巨噬细胞（TRF-M）的辅助性T细胞替代因子的生物学活性进行了比较。TRF-T在T细胞耗竭的脾细胞培养物中可刺激对异源红细胞抗原（SRBC和BRBC）的免疫反应，但在巨噬细胞耗竭的脾细胞培养物中则无此作用。TRF-M在T细胞耗竭和巨噬细胞耗竭的脾细胞培养物中均可刺激免疫反应。在脂多糖刺激活化巨噬细胞培养物释放TRF-M的条件下，TRF-T对TRF-M的产生没有影响。因此，TRF-T似乎不是通过刺激巨噬细胞释放TRF-M来发挥作用的。在巨噬细胞耗竭的脾细胞培养物中，当将饱和浓度的TRF-T和TRF-M混合在一起时，对红细胞抗原免疫反应的诱导表现出显著的协同作用。TRF-M和TRF-T协同作用的动力学与TRF-M对TRF-T敏感B细胞产生的影响一致。
