Thoman M L, Morgan E L, Weigle W O
J Immunol. 1980 Oct;125(4):1630-3.
Fc fragments of human gamma-globulin stimulate T cell-dependent polyclonal antibody responses in murine splenic B cells. The T cell signal can be delivered by a soluble T cell-replacing factor, derived by concanavalin A stimulation of spleen cells. TRF interacts directly with responding B cells and appears to provide the second, differentiative, trigger that initiates the polyclonal response. TRF is maximally active even if added at 24 hr after Fc and does not alter the response kinetics. Although macrophages are requird for the polyclonal activation of B cells in intact spleen cell cultures, they are not necessary for the delivery of the TRF signal to B cells.
人γ-球蛋白的Fc片段可刺激鼠脾B细胞中依赖T细胞的多克隆抗体反应。T细胞信号可由一种可溶性T细胞替代因子传递，该因子通过刀豆球蛋白A刺激脾细胞获得。TRF直接与反应性B细胞相互作用，似乎提供了启动多克隆反应的第二个、分化性的触发信号。即使在Fc加入后24小时添加，TRF仍具有最大活性，且不改变反应动力学。虽然在完整脾细胞培养物中B细胞的多克隆激活需要巨噬细胞，但将TRF信号传递给B细胞则不需要巨噬细胞。
