Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, PR China.
School of Medical Imaging, Tianjin Medical University, Tianjin, 300203, PR China.
Biomaterials. 2019 Oct;217:119332. doi: 10.1016/j.biomaterials.2019.119332. Epub 2019 Jul 2.
Luminescent porous materials have been widely used in biosensing, bioimaging and drug delivery by virtue of the special porous structure and luminescent property. The main obstacle for the application in biosensing and bioimaging is the background interference of external irradiation. Herein, we report a background interference-free persistent luminescent metal-organic framework (PLMOF) with persistent luminescent near infrared (NIR) luminescence for tumor site activated persistent luminescence imaging. The PLMOF (PLNPs@ZIF-8) was prepared by in-situ growth of MOF on the persistent luminescent nanoparticles (PLNPs) via a surface adsorption induced self-assembly method. The PLMOF possessed NIR persistent luminescence and renewable NIR luminescence and thus enabled deep-tissue and long-term imaging without external excitation. Specifically, the PLMOF showed acidic tumor site activated persistent luminescence for in vitro and in vivo tumor imaging, which was also help to reduce the background interference. The mechanism of acidic activation was attributed to the protonation of imidazole that induces disassembly of ZIF-8. In addition, the PLMOF presented a high anti-cancer drug loading capacity, acidity-responsive drug release behavior, and significant anti-tumor effect. All these results indicate that our PLMOF can serve as a promising theranostic platform for precision medicine.
具有发光多孔结构的材料由于其特殊的多孔结构和发光性能，被广泛应用于生物传感、生物成像和药物输送中。在生物传感和生物成像中的主要应用障碍是外部辐照的背景干扰。在此，我们报道了一种背景无干扰的持续发光金属有机骨架（PLMOF），具有持续发光的近红外（NIR）发光，可用于肿瘤部位激活的持续发光成像。PLMOF（PLNPs@ZIF-8）是通过表面吸附诱导自组装方法，在持续发光纳米颗粒（PLNPs）上原位生长 MOF 制备得到的。PLMOF 具有 NIR 持续发光和可再生 NIR 发光，因此可以在没有外部激发的情况下进行深层组织和长期成像。具体而言，PLMOF 表现出酸性肿瘤部位激活的持续发光，可用于体外和体内肿瘤成像，这也有助于减少背景干扰。酸性激活的机制归因于导致 ZIF-8 解组装的咪唑质子化。此外，PLMOF 还具有高载药能力、酸响应性药物释放行为和显著的抗肿瘤效果。所有这些结果表明，我们的 PLMOF 可以作为一种有前途的精准医疗治疗平台。
