Bale M J, Fry J C, Day M J
Department of Applied Biology, University of Wales Institute of Science and Technology, Cardiff, UK.
J Gen Microbiol. 1987 Nov;133(11):3099-107. doi: 10.1099/00221287-133-11-3099.
A naturally occurring mercury-resistance, conjugative plasmid, designated pQM1, was isolated from a bacterial population on the surface of stones from a river using Pseudomonas aeruginosa as a recipient. This was a narrow-host-range plasmid [IncP-13; 165 MDa; Tra+, Hgr, fluorescein mercuric acetater, merbrominr, Phi(E79), UVr] confined to some Pseudomonas spp. It was used to demonstrate transfer between bacteria on stones in laboratory microcosm experiments and in situ. Transfer occurred (3.3 X 10(-1) to 6.8 X 10(-9) per recipient) at all the temperatures used (6-20 degrees C), although frequencies were lower in the cold. Nutrient status also affected transfer frequency, rich conditions promoting transfer. The presence of competing bacteria in the natural epilithon lowered transfer frequencies, but when unscrubbed stones were heat treated, transfer was enhanced, perhaps because of nutrient release from the heated epilithon.
从一条河流中石头表面的细菌群体中分离出一种天然存在的抗汞接合质粒，命名为pQM1，以铜绿假单胞菌作为受体。这是一种窄宿主范围质粒[IncP - 13；165 MDa；Tra +，Hgr，荧光素汞乙酸盐，汞溴红，Phi(E79)，UVr]，局限于某些假单胞菌属物种。在实验室微观实验和原位实验中，它被用于证明石头上细菌之间的转移。在所有使用的温度下（6 - 20摄氏度）都发生了转移（每个受体的转移频率为3.3×10(-1)至6.8×10(-9)），尽管在低温下频率较低。营养状况也影响转移频率，丰富的条件促进转移。天然石表微生物中竞争细菌的存在降低了转移频率，但当未擦洗的石头经过热处理时，转移增强，这可能是因为加热的石表微生物释放了营养物质。
