Nghiem Y, Cabrera M, Cupples C G, Miller J H
Molecular Biology Institute, University of California, Los Angeles 90024.
Proc Natl Acad Sci U S A. 1988 Apr;85(8):2709-13. doi: 10.1073/pnas.85.8.2709.
We have used a strain with an altered lacZ gene, which reverts to wild type via only certain transversions, to detect transversion-specific mutators in Escherichia coli. Detection relied on a papillation technique that uses a combination of beta-galactosides to reveal blue Lac+ papillae. One class of mutators is specific for the G.C----T.A transversion as determined by the reversion pattern of a set of lacZ mutations and by the distribution of forward nonsense mutations in the lacI gene. The locus responsible for the mutator phenotype is designated mutY and maps near 64 min on the genetic map of E. coli. The mutY locus may act in a similar but reciprocal fashion to the previously characterized mutT locus, which results in A.T----C.G transversions.
我们使用了一个 lacZ 基因发生改变的菌株，该菌株仅通过某些颠换回复为野生型，以检测大肠杆菌中特定于颠换的诱变剂。检测依赖于一种菌斑技术，该技术使用β-半乳糖苷的组合来揭示蓝色 Lac+ 菌斑。通过一组 lacZ 突变的回复模式以及 lacI 基因中正向无义突变的分布确定，一类诱变剂对 G.C----T.A 颠换具有特异性。负责诱变剂表型的基因座被命名为 mutY，位于大肠杆菌遗传图谱上约 64 分钟处。mutY 基因座的作用方式可能与先前表征的 mutT 基因座类似但相反，mutT 基因座会导致 A.T----C.G 颠换。
