Ramain P, Giangrande A, Richards G, Bellard M
Laboratoire de Génétique Moléculaire des Eucaryotes du Centre National de la Recherche Scientifique, Faculté de Médecine, Strasbourg, France.
Proc Natl Acad Sci U S A. 1988 Apr;85(8):2718-22. doi: 10.1073/pnas.85.8.2718.
We have undertaken chromatin studies on transformed Drosophila strains carrying DNA sequences modified in the region of the DNase I (EC 3.1.4.5)-hypersensitive sites -750 and -600 base pairs upstream from the Sgs3 start site. Although both sites are developmentally specific, modifications in the -750 site have little or no effect on Sgs3-encoded transcript levels, whereas either deletion or replacement of sequences at the -600 site causes an important reduction in transcript levels. The element associated with the -600 site enhances Sgs3 transcription when displaced with respect to the start site. This combined approach has defined sequence elements necessary both for normal transcript levels as well as the chromatin structure characteristic of Sgs3 activity in vivo.
我们对携带在Sgs3起始位点上游-750和-600碱基对处经修饰的DNA序列的转化果蝇品系进行了染色质研究。尽管这两个位点都具有发育特异性，但-750位点的修饰对Sgs3编码的转录本水平几乎没有影响，而-600位点的序列缺失或替换会导致转录本水平显著降低。与-600位点相关的元件在相对于起始位点移位时会增强Sgs3转录。这种综合方法确定了正常转录本水平以及体内Sgs3活性特征性染色质结构所必需的序列元件。
