Yamamoto M, Shimokata K, Nagura H
Department of First Internal Medicine, Nagoya University School of Medicine, Japan.
Virchows Arch A Pathol Anat Histopathol. 1988;412(5):479-86. doi: 10.1007/BF00750582.
The existence of a subpopulation of human vascular endothelial cells (EC) has been demonstrated in the liver with the aid of immunohistochemical techniques. In this study, we investigated the antigenic and functional properties of the vascular EC in human lung. Alveolar capillary EC shared antigens with a peripheral blood monocyte/macrophage subset capable of presenting soluble antigens and triggering autologous mixed lymphocyte reactions. That is to say that the alveolar capillary EC were HLA-DR+, OKM1-, and OKM5+. In addition, these EC frequently expressed interleukin-1. These facts suggest that alveolar capillary EC may play an important role in immunological responses in the lung. The antigens were, however, absent or only faintly visible on the vascular EC of medium and small vessels. In contrast, Factor VIII/von Willebrand factor antigen (FVIIIRAg), which is produced in vascular EC was heavily stained in the EC of medium and small vessels, but only weakly stained in the alveolar capillary EC. These immunohistochemical findings suggest that in different anatomical compartments in the lung vascular EC express phenotypic properties heterogeneously. They may play differing biological roles or serve different immunological functions in normal and pathological states in the lung.
借助免疫组织化学技术已证实在肝脏中存在人类血管内皮细胞（EC）亚群。在本研究中，我们调查了人类肺脏中血管内皮细胞的抗原和功能特性。肺泡毛细血管内皮细胞与能够呈递可溶性抗原并引发自体混合淋巴细胞反应的外周血单核细胞/巨噬细胞亚群共享抗原。也就是说，肺泡毛细血管内皮细胞HLA - DR阳性、OKM1阴性、OKM5阳性。此外，这些内皮细胞经常表达白细胞介素 - 1。这些事实表明肺泡毛细血管内皮细胞可能在肺脏的免疫反应中起重要作用。然而，这些抗原在中小血管的血管内皮细胞上不存在或仅微弱可见。相反，在血管内皮细胞中产生的因子VIII /血管性血友病因子抗原（FVIIIRAg）在中小血管的内皮细胞中染色很深，但在肺泡毛细血管内皮细胞中仅微弱染色。这些免疫组织化学结果表明，在肺脏的不同解剖区域，血管内皮细胞异质性地表达表型特性。它们在肺脏的正常和病理状态下可能发挥不同的生物学作用或具有不同的免疫功能。
