Nutrition and Food Science Area, Faculty of Pharmacy , University of Valencia , Avenida Vicente Andrés Estellés/n , Burjassot, Valencia 46100 , Spain.
J Agric Food Chem. 2020 Feb 19;68(7):1862-1870. doi: 10.1021/acs.jafc.9b03025. Epub 2019 Jul 18.
Plant sterol (PS) (1 g/100 mL) enriched milk-based fruit beverages with or without galactooligosaccharides (GOS) (1.8 g/100 mL) were used in differentiated Caco-2 cells. Their potential cytopreventive effect against oxidative stress induced by cholesterol oxidation products (COPs) and their anti-inflammatory properties were evaluated. Preincubation (24 h) with bioaccessible fractions from beverages without and with GOS (MfB and MfB-G) completely prevented the COPs (60 μM/4 h) induced oxidative stress independent to GOS presence with exception to calcium influx and GSH content, where a partial protective effect was observed. Besides, MfB produced a significant ( < 0.05) reduction of IL-8 (40%) and IL-6 (50%) after IL-1β-induction (1 ng/mL/24 h) through the inhibition of NF-κB p65 translocation into the nucleus (16%) compared to control cells, while GOS presence compromised their anti-inflammatory effect. Therefore, PS-enriched milk-based fruit beverage could be an interesting strategy to prevent intestinal injury produced by COPs and to attenuate the pro-inflammatory process in intestinal human diseases. GOS addition had no extra beneficial antioxidant effect and even reduced their anti-inflammatory properties.
用含有或不含有半乳糖寡糖（GOS）（1.8 g/100 mL）的植物甾醇（PS）（1 g/100 mL）强化牛奶基水果饮料处理分化的 Caco-2 细胞。评估它们对胆固醇氧化产物（COPs）诱导的氧化应激的潜在细胞保护作用及其抗炎特性。用不含和含 GOS 的饮料的可生物利用部分（MfB 和 MfB-G）进行 24 小时预孵育完全阻止了 COPs（60 μM/4 h）诱导的氧化应激，与 GOS 存在无关，但观察到钙内流和 GSH 含量的部分保护作用。此外，与对照细胞相比，MfB 通过抑制 NF-κB p65 向核内易位（16%），使 IL-1β 诱导（1 ng/mL/24 h）后 IL-8（40%）和 IL-6（50%）的产生显著降低（<0.05），而 GOS 的存在则损害了其抗炎作用。因此，富含 PS 的牛奶基水果饮料可能是预防 COPs 引起的肠道损伤和减轻肠道人类疾病中促炎过程的一种有趣策略。添加 GOS 没有额外的有益的抗氧化作用，甚至降低了它们的抗炎特性。
