矮杨梅叶提取物在动物细胞培养中的抗病毒活性。
Virucidal activity of Garcinia parvifolia leaf extracts in animal cell culture.
机构信息
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Department of Cell Biology and Physiology, School of Medicine, University North Carolina, Chapel Hill, NC, 27599, USA.
出版信息
BMC Complement Altern Med. 2019 Jul 10;19(1):169. doi: 10.1186/s12906-019-2586-5.
BACKGROUND
Garcinia species contain bioactive compounds such as flavonoids, xanthones, triterpernoids, and benzophenones with antibacterial, antifungal, anti-inflammatory, and antioxidant activities. In addition, many of these compounds show interesting biological properties such as anti-human immunodeficiency virus activity. Garcinia parvifolia is used in traditional medicine. Currently, the antiviral activity of G. parvifolia is not known.
METHODS
This study was conducted to determine the effects of ethyl acetate (45 L Ea), ethanol (45 L Et), and hexane (45 L H) leaf extracts of G. parvifolia on the infectivity of pseudorabies virus (PrV) in Vero cells. The antiviral effects of the extracts were determined by cytopathic effect (CPE), inhibition, attachment, and virucidal assays.
RESULTS
The 50% cytotoxicity concentration (CC) values obtained were 237.5, 555.0, and < 1.25 μg/mL for 45 L Ea, 45 L Et, and 45 L H, respectively. The 45 L Ea showed the greatest viral inhibition potency of 75% at 125 μg/mL. Both 45 L Ea and 45 l Et caused 100% residual viral inhibition at 250 μg/mL. The selectivity index values for 45 L Ea, 45 L Et, and 45 L H were 2.65, 1.75, and 0.10 showing that 45 L Ea had the greatest antiviral activity among the three extracts.
CONCLUSION
This study showed that ethyl acetate is the best solvent to be used to obtain extract from G. parvifolia leaves with potent antiviral activities.
背景
藤黄属植物含有生物活性化合物，如类黄酮、紫檀烷、三萜和二苯甲酮，具有抗菌、抗真菌、抗炎和抗氧化活性。此外，许多这些化合物表现出有趣的生物学特性，如抗人类免疫缺陷病毒活性。小花藤黄在传统医学中被使用。目前，小花藤黄的抗病毒活性尚未可知。
方法
本研究旨在确定小花藤黄叶的乙酸乙酯（45L Ea）、乙醇（45L Et）和正己烷（45L H）提取物对伪狂犬病病毒（PrV）在 Vero 细胞中的感染性的影响。提取物的抗病毒作用通过细胞病变效应（CPE）、抑制、附着和病毒杀灭试验来确定。
结果
获得的 50%细胞毒性浓度（CC）值分别为 237.5、555.0 和 <1.25μg/mL，对于 45L Ea、45L Et 和 45L H。在 125μg/mL 时，45L Ea 显示出最大的病毒抑制效力为 75%。在 250μg/mL 时，45L Ea 和 45L Et 均导致 100%残留病毒抑制。45L Ea、45L Et 和 45L H 的选择性指数值分别为 2.65、1.75 和 0.10，表明 45L Ea 在三种提取物中具有最大的抗病毒活性。
结论
本研究表明，乙酸乙酯是从小花藤黄叶中获得具有潜在抗病毒活性提取物的最佳溶剂。
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