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板蓝根提取物对白血病细胞的细胞毒性及对伪狂犬病病毒的抗病毒作用。

The cytotoxicity to leukemia cells and antiviral effects of Isatis indigotica extracts on pseudorabies virus.

作者信息

Hsuan Shih-Ling, Chang Shih-Chieh, Wang Sheng-Yang, Liao Tien-Ling, Jong Ting-Ting, Chien Maw-Sheng, Lee Wei-Cheng, Chen Shih-Shiung, Liao Jiunn-Wang

机构信息

Graduate Institute of Veterinary Pathobiology and Department of Veterinary Medicine, National Chung-Hsing University, 250, Kuo-Kuang Road, Taichung, 402, Taiwan, ROC.

出版信息

J Ethnopharmacol. 2009 May 4;123(1):61-7. doi: 10.1016/j.jep.2009.02.028. Epub 2009 Mar 4.

DOI:10.1016/j.jep.2009.02.028
PMID:19429341
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7126793/
Abstract

AIM OF THE STUDY

Isatis indigotica (I. indigotica), Cruciferae, has been used in Chinese medicine for anti-leukemia and anti-severe acute respiratory syndrome (SARS). The aim of this study was to evaluate the cytotoxicity of Isatis indigotica extracts on human leukemia cell line (HL-60) and the antiviral activity on swine pseudorabies virus (PrV) in in vitro assays.

MATERIALS AND METHODS

Extracts and derived fractions of Isatis indigotica were prepared from root (R) and leaf (L) using methanol (M), ethyl acetate (E) and distilled water (D). The cytotoxic effect of extracts on swine peripheral blood mononuclear cells (PBMCs) and HL-60 was assessed by MTT method. The cytopathic effect (CPE) reduction, plaque reduction and inhibition assays on viral replication, and virucidal activity were further conducted to investigate the anti-PrV activity.

RESULTS

Indirubin, one of the biological active compounds of Isatis indigotica, had the most significant cytotoxicity on HL-60 cells and inhibitory effect on PrV replication. Extracts from roots and leaves of Isatis indigotica also presented CPE inhibition either before or after infection of PrV on porcine kidney (PK-15) cells. Leaf extracts had better virucidal activity than roots, and ethyl acetate extracts exhibited the highest efficacy among extracts tested.

CONCLUSION

Isatis indigotica posses a valuable virucidal effect in disease control of pseudorabies virus infection in swine.

摘要

研究目的

十字花科植物菘蓝在中国医学中被用于抗白血病和抗严重急性呼吸综合征（SARS）。本研究的目的是在体外试验中评估菘蓝提取物对人白血病细胞系（HL-60）的细胞毒性以及对猪伪狂犬病病毒（PrV）的抗病毒活性。

材料与方法

使用甲醇（M）、乙酸乙酯（E）和蒸馏水（D）从菘蓝的根（R）和叶（L）中制备提取物及其衍生组分。采用MTT法评估提取物对猪外周血单个核细胞（PBMCs）和HL-60的细胞毒性作用。通过细胞病变效应（CPE）降低、蚀斑减少和病毒复制抑制试验以及杀病毒活性试验进一步研究抗PrV活性。

结果

靛玉红是菘蓝的生物活性化合物之一，对HL-   60细胞具有最显著的细胞毒性，并对PrV复制具有抑制作用。菘蓝根和叶的提取物在PrV感染猪肾（PK-15）细胞之前或之后均表现出CPE抑制作用。叶提取物的杀病毒活性优于根提取物，并且乙酸乙酯提取物在所测试的提取物中表现出最高的效力。

结论

菘蓝在猪伪狂犬病病毒感染的疾病控制中具有重要的杀病毒作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1de/7126793/ca5c8de120fe/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1de/7126793/d7216bdf06b9/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1de/7126793/a9c41c6f0b20/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1de/7126793/536beb81840c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1de/7126793/c2c9d860d0a6/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1de/7126793/ca5c8de120fe/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1de/7126793/d7216bdf06b9/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1de/7126793/a9c41c6f0b20/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1de/7126793/536beb81840c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1de/7126793/c2c9d860d0a6/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1de/7126793/ca5c8de120fe/gr5.jpg

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