基于 Ag 掺杂碳点催化扩增和适体调控的 Pb(II)双模式 SERS 和 RRS 分析平台。

A dual-model SERS and RRS analytical platform for Pb(II) based on Ag-doped carbon dot catalytic amplification and aptamer regulation.

机构信息

Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, 541004, China.

出版信息

Sci Rep. 2019 Jul 10;9(1):9991. doi: 10.1038/s41598-019-46426-y.

Several carbon dots doping with diferent elements (Ca, Ag, Au) were fabricated and their catalytic properties had been investigated in this paper. It was found that the Ag-doped carbon dots (CD) had played a role of mimic enzyme on the reaction of HAuCl-HO and generated nanogold particles with surface enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) effects. The aptamer (Apt) can be adsorbed on the CD surface and cause the catalysis weakening. When the target Pb(II) was added, it would combine with the Apt to produce firm complexes Pb-Apt and desorb CD, which caused its catalytic effect restore. The formed nanogold had a strong RRS peak (at 375 nm) and a high SERS peak (at 1615 cm) in the presence of molecular probe (Victoria blue B, VBB). The dual-model signals of SERS and RRS increased linearly with Pb(II) concentration increase within the scope of 0.006-0.46 μmol/L and 0.01-0.46 μmol/L. And their detection limits respectively were 0.0032 μmol/L and 0.0048 μmol/L Pb(II).

本文制备了几种不同元素（Ca、Ag、Au）掺杂的碳点，并研究了它们的催化性能。研究发现，Ag 掺杂的碳点（CD）在 HAuCl-HO 反应中模拟酶的作用，生成具有表面增强拉曼散射（SERS）和共振瑞利散射（RRS）效应的纳米金颗粒。适配体（Apt）可以吸附在 CD 表面，导致催化作用减弱。当加入目标 Pb（II）时，它会与 Apt 结合生成稳定的复合物 Pb-Apt 并解吸 CD，从而恢复其催化作用。形成的纳米金在分子探针（维多利亚蓝 B，VBB）存在下具有很强的 RRS 峰（在 375nm 处）和高 SERS 峰（在 1615cm 处）。在 0.006-0.46 μmol/L 和 0.01-0.46 μmol/L 范围内，SERS 和 RRS 的双模式信号随 Pb（II）浓度的增加呈线性增加，其检测限分别为 0.0032 μmol/L 和 0.0048 μmol/L Pb（II）。