掺杂N/Ag碳点催化放大表面增强拉曼散射策略用于啶虫脒与3,3'-二甲基联苯-4,4'-二胺氧化反应的适配体耦合

Doped N/Ag Carbon Dot Catalytic Amplification SERS Strategy for Acetamiprid Coupled Aptamer with 3,3'-Dimethylbiphenyl-4,4'-diamine Oxidizing Reaction.

作者信息

Feng Xiaozhen, Li Chongning, Liang Aihui, Luo Yanghe, Jiang Zhiliang

机构信息

Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541004, China.

School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, China.

出版信息

Nanomaterials (Basel). 2019 Mar 25;9(3):480. doi: 10.3390/nano9030480.

DOI:10.3390/nano9030480

PMID:30934552

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6474095/

Abstract

The as-prepared co-doped N/Ag carbon dot (CD) has strong catalysis of H₂O₂ oxidation of 3,3'-dimethylbiphenyl-4,4'-diamine (DBD). It forms an oxidation product (DBD) with surface-enhanced Raman scattering (SERS) activity at 1605 cm in the silver nanosol substrate, and a CD catalytic amplification with SERS analytical platform can be structured based on aptamer (Apt) with the DBD oxidizing reaction. For example, the aptamer (Apt) of acetamiprid (ACT) can be adsorbed on the surface of CD, resulting in inhibited catalytic activity, the reduced generation of DBD, and a weakened SERS intensity. When the target molecule ACT was added, it formed a stable Apt-ACT complex and free CD that restored catalytic activity and linearly enhanced the SERS signal. Based on this, we proposed a new quantitative SERS analysis method for the determination of 0.01⁻1.5 μg ACT with a detection limit of 0.006 μg/L.

摘要

所制备的共掺杂N/Ag碳点（CD）对3,3'-二甲基联苯-4,4'-二胺（DBD）的H₂O₂氧化具有很强的催化作用。它在银纳米溶胶底物中形成了在1605 cm处具有表面增强拉曼散射（SERS）活性的氧化产物（DBD），并且可以基于DBD氧化反应的适体（Apt）构建具有SERS分析平台的CD催化放大体系。例如，啶虫脒（ACT）的适体（Apt）可以吸附在CD表面，导致催化活性受到抑制，DBD生成减少，SERS强度减弱。当加入目标分子ACT时，它形成稳定的Apt-ACT复合物并释放出恢复催化活性并线性增强SERS信号的游离CD。基于此，我们提出了一种新的定量SERS分析方法，用于测定0.01⁻1.5 μg的ACT，检测限为0.006 μg/L。