Barragán-Méndez Cristina, Sobrino Ignacio, Marín-Rincón Adrián, Fernández-Boo Sergio, Costas Benjamin, Mancera Juan Miguel, Ruiz-Jarabo Ignacio
Department of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar, Universidad de Cádiz, Cádiz, Spain.
Instituto Español de Oceanografía, Centro Oceanográfico de Cádiz, Cádiz, Spain.
Front Physiol. 2019 Jun 25;10:784. doi: 10.3389/fphys.2019.00784. eCollection 2019.
Several Octopodidae species have a great potential for the diversification of worldwide aquaculture. Unfortunately, the lack of stress-related biomarkers in this taxon results an obstacle for its maintenance in conditions where animal welfare is of paramount relevance. In this study, we made a first approach to uncover physiological responses related to fishing capture in , , and . Captured octopus from all three species were individually maintained in an aquaculture system onboard of oceanographic vessel in south-western waters of Europe. Haemolymph plasma and muscle were collected in animals at the moment of capture, and recovery was evaluated along a time-course of 48 h in spp., and 24 h for . Survival rates of these species captured in spring and autumn were evaluated. Physiological parameters such as plasma pH, total CO, peroxidase activity, lysozyme, hemocyanin, proteases, pro-phenoloxidase, anti-proteases, free amino acids, lactate and glucose levels, as well as muscle water percentage, free amino acids, lactate, glycogen and glucose values were analyzed. The immune system appears to be compromised in these species due to capture processes, while energy metabolites were mobilized to face the acute-stress situation, but recovery of all described parameters occurs within the first 24 h after capture. Moreover, this situation exerts hydric balance changes, as observed in the muscle water, being these responses depending on the species assessed. In conclusion, three Octopodidae species from south-western waters of Europe have been evaluated for stress-related biomarkers resulting in differentiated mechanisms between species. This study may pave the way to further study the physiology of stress in adult octopuses and develop new methodologies for their growth in aquaculture conditions.
几种蛸科物种在全球水产养殖多样化方面具有巨大潜力。不幸的是，该分类群中缺乏与应激相关的生物标志物，这成为在动物福利至关重要的条件下对其进行养殖的障碍。在本研究中，我们首次尝试揭示与捕捞相关的生理反应，涉及[具体物种1]、[具体物种2]和[具体物种3]。从这三个物种捕获的章鱼被单独饲养在欧洲西南部海域一艘海洋学船上的水产养殖系统中。在捕获时采集动物的血淋巴血浆和肌肉样本，并在[具体物种1]中沿着48小时的时间进程评估恢复情况，对于[具体物种2]则评估24小时的恢复情况。评估了这些物种在春季和秋季捕获后的存活率。分析了诸如血浆pH值、总二氧化碳、过氧化物酶活性、溶菌酶、血蓝蛋白、蛋白酶、前酚氧化酶、抗蛋白酶、游离氨基酸、乳酸和葡萄糖水平等生理参数，以及肌肉水分百分比、游离氨基酸、乳酸、糖原和葡萄糖值。由于捕获过程，这些物种的免疫系统似乎受到损害，同时能量代谢物被调动以应对急性应激情况，但所有上述参数在捕获后的头24小时内恢复。此外，这种情况会导致水分平衡变化，如在肌肉水分中观察到的那样，这些反应取决于所评估的物种。总之，对来自欧洲西南部海域的三种蛸科物种进行了与应激相关生物标志物的评估，结果表明不同物种之间存在不同的机制。这项研究可能为进一步研究成年章鱼的应激生理学以及开发水产养殖条件下其生长的新方法铺平道路。
