Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; College of Life Science, Shandong Agricultural University, Taian 271018, PR China.
College of Life Science, Shandong Agricultural University, Taian 271018, PR China.
Int J Biol Macromol. 2019 Sep 15;137:1130-1140. doi: 10.1016/j.ijbiomac.2019.07.044. Epub 2019 Jul 8.
This work investigated the protective effects of Ganoderma applanatum residue polysaccharides (GRP) on the CCl-induced hepatotoxicity. The results indicated that GRP showed significantly effects on preventing the increase of AST, ALT and ALP levels in serum, elevating the activities of SOD, GSH-Px and CAT, decreasing the contents of MDA and LPO, and reducing the CYP2E1 and TGF-β concentrations in CCl-induced mice, respectively. Meanwhile, the levels of TNF-α and IL-6 were significantly decreased, while the value of IL-10 was increased by GRP treatment. Besides, the western blot assay showed the IκBα expressions were significantly increased and the p-p65 was decreased by the treatment with GRP. The characterizations indicated that the GRP was heteropolysaccharide with lower molecular weights and α-furanoside residues. These results demonstrated that GRP might be a potential material for drug and functional food development against chemical hepatic injury.
这项工作研究了灵芝菌渣多糖（GRP）对 CCl 诱导的肝毒性的保护作用。结果表明，GRP 对预防血清中 AST、ALT 和 ALP 水平的升高、提高 SOD、GSH-Px 和 CAT 的活性、降低 MDA 和 LPO 的含量以及降低 CCl 诱导的小鼠 CYP2E1 和 TGF-β浓度具有显著作用。同时，GRP 处理可显著降低 TNF-α和 IL-6 的水平，而增加 IL-10 的水平。此外，Western blot 分析表明，GRP 处理可显著增加 IκBα 的表达，降低 p-p65 的表达。这些特征表明，GRP 是一种具有较低分子量和α-呋喃糖苷残基的杂多糖。这些结果表明，GRP 可能是一种用于开发针对化学性肝损伤的药物和功能性食品的潜在物质。
