Hossain Mohammad Shahadat, Barua Anik, Tanim Mohammad Akramul Hoque, Hasan Mohammad Sharif, Islam Mohammad Jahedul, Hossain Md Rabiul, Emon Nazim Uddin, Hossen S M Moazzem
Department of Pharmacy Faculty of Biological Science University of Chittagong Chattogram Bangladesh.
Department of Biochemistry and Biotechnology University of Science and Technology Chittagong Chattogram Bangladesh.
Food Sci Nutr. 2021 Jun 24;9(8):4364-4374. doi: 10.1002/fsn3.2407. eCollection 2021 Aug.
This study was undertaken to evaluate the antidiabetic, hypolipidemic, and hepatoprotective effects of methanol and aqueous extracts of (MEGA, AEGA) in alloxan-induced diabetic rats. The antidiabetic study was implemented by the induction of alloxan to the rats. The analysis of the hypolipidemic and liver-protective effects of fungus extracts was studied by estimating the lipid profile and the liver marker enzymes. Besides, in silico screening of the compounds of has been incorporated thus to check the binding affinity of compounds and enzymes affinity. The Discovery Studio 2020, UCSF Chimera, and PyRx AutoDock Vina have been used to implement the docking analysis. Nine days of oral feeding of MEGA and AEGA of resulted in a significant ( < .001) reduction in blood glucose, lipid profile, and liver marker enzymes. Besides, Myrocin C scored the highest score in the docking study. The biological and computational approaches suggested the MEGA and AEGA could be a potential source for antidiabetic, hypolipidemic, and hepatoprotective effects.
本研究旨在评估巨型曲霉甲醇提取物（MEGA）和水提取物（AEGA）对四氧嘧啶诱导的糖尿病大鼠的抗糖尿病、降血脂和肝脏保护作用。通过给大鼠注射四氧嘧啶来进行抗糖尿病研究。通过估计血脂谱和肝脏标志物酶来研究真菌提取物的降血脂和肝脏保护作用。此外，还进行了巨型曲霉化合物的计算机模拟筛选，以检查化合物的结合亲和力和酶亲和力。使用Discovery Studio 2020、UCSF Chimera和PyRx AutoDock Vina进行对接分析。连续九天口服巨型曲霉的MEGA和AEGA可使血糖、血脂谱和肝脏标志物酶显著降低（P<0.001）。此外，Myrocin C在对接研究中得分最高。生物学和计算方法表明，MEGA和AEGA可能是具有抗糖尿病、降血脂和肝脏保护作用的潜在来源。
