School of Life Sciences and School of Physical Education, Yunnan Normal University, Kunming 650500, China; Engineering Research Center of Sustainable Development and Utilization of Biomass, MOE, Kunming 650500, China; Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, China.
School of Agriculture and Laboratory of Ecology and Evolutionary Biology, Yunnan University, Kunming 650504, China.
Exp Gerontol. 2019 Oct 1;125:110651. doi: 10.1016/j.exger.2019.110651. Epub 2019 Jul 8.
Bidens pilosa L., a herbal medicine, is rich in flavonoids, but its anti-aging effect on neurocognitive functions is not well understood. In the present study, we investigated the effects of ethanol extract from Bidens pilosa L. (EEBP) on spontaneous activity, learning and memory in aged rats.
Forty aged (21.90 ± 0.22 months) and 10 young (10 weeks) adult male Sprague-Dawley rats were divided into 5 groups, which were respectively treated orally with 0 mg/kg (young and aged control), 25 mg/kg, 50 mg/kg and 100 mg/kg of EEBP for 30 days consecutively. Then, the animals were examined with open-field, passive avoidance and Morris water maze tasks.
In the open-field task, compared with the aged control, the EEBP animals exhibited more rearing (50 mg/kg, P < 0.01) and urination (50 mg/kg, P < 0.01), but less defecation (P < 0.05). In the passive avoidance task, the retention latencies were longer than those in the training phase in all other groups (P < 0.01) except the aged control (P > 0.05). Compared with the young control, the retention latency of the aged control decreased (P < 0.01), but that of the EEBP animals increased again (P < 0.05 vs. aged control). In the Morris water maze, the EEBP animals had shorter latency (100 mg/kg) and had more crossing times (25 mg/kg) in seeking the platform position (P < 0.05, vs. aged control).
The results suggested that EEBP could affect the spontaneous activity and improve memory in aged animals and could have potential advantages for cognition improvement in aged populations.
鬼针草 L. 是一种草药，富含类黄酮，但它对神经认知功能的抗衰老作用尚不清楚。在本研究中，我们研究了鬼针草 L. 乙醇提取物（EEBP）对老年大鼠自发活动、学习和记忆的影响。
40 只老年（21.90±0.22 个月）和 10 只成年雄性 Sprague-Dawley 大鼠被分为 5 组，分别连续口服 0mg/kg（年轻和老年对照组）、25mg/kg、50mg/kg 和 100mg/kg 的 EEBP 30 天。然后，动物通过旷场、被动回避和 Morris 水迷宫任务进行检查。
在旷场任务中，与老年对照组相比，EEBP 动物表现出更多的举尾（50mg/kg，P<0.01）和排尿（50mg/kg，P<0.01），但排便减少（P<0.05）。在被动回避任务中，除老年对照组外（P>0.05），所有其他组的潜伏期均长于训练阶段（P<0.01）。与年轻对照组相比，老年对照组的潜伏期缩短（P<0.01），但 EEBP 动物的潜伏期再次延长（P<0.05 与老年对照组相比）。在 Morris 水迷宫中，EEBP 动物潜伏期较短（100mg/kg），寻找平台位置的穿越次数较多（25mg/kg）（P<0.05，与老年对照组相比）。
结果表明，EEBP 可影响老年动物的自发活动和改善记忆，并可能对老年人群的认知改善具有潜在优势。
