Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
Bioresour Technol. 2019 Oct;290:121715. doi: 10.1016/j.biortech.2019.121715. Epub 2019 Jun 27.
Bacterial cellulose (BC) based composites have been widely studied in the biomedical field. In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system. The concentration of HA secreted by L. lactis was controlled through adjusting the constant feed rate of glucose. The dynamic growth of the strains revealed that L. lactis was mainly growing within 48 h while G. hansenii started to grow after 48 h. XRD analysis indicated the presence of HA would not affect the crystallinity of cellulose but increase the crystalline sizes. The FESEM images showed that more ribbons within the width of 20-40 nm and larger ribbons between 180 and 360 nm were observed in BC/HA. The strain at break and the water holding capacity of BC/HA increased with the concentration of HA.
基于细菌纤维素 (BC) 的复合材料在生物医学领域得到了广泛研究。在本研究中，通过在新型两容器循环系统中共同培养 Gluconacetobacter hansenii ATCC 23769 和 Lactococcus lactis APJ3，直接制备了膜状的 BC/HA（透明质酸）纳米复合材料。通过调整葡萄糖的恒速进料率来控制 L. lactis 分泌的 HA 的浓度。菌株的动态生长表明，L. lactis 主要在 48 小时内生长，而 G. hansenii 在 48 小时后开始生长。XRD 分析表明，HA 的存在不会影响纤维素的结晶度，但会增加结晶尺寸。FESEM 图像显示，在 BC/HA 中观察到更多宽度在 20-40nm 之间的带状物和宽度在 180-360nm 之间的较大带状物。BC/HA 的断裂应变和持水能力随 HA 浓度的增加而增加。
