Rodríguez-Tomàs Elisabet, Murcia Mauricio, Arenas Meritxell, Arguís Mònica, Gil Miriam, Amigó Núria, Correig Xavier, Torres Laura, Sabater Sebastià, Baiges-Gayà Gerard, Cabré Noemí, Luciano-Mateo Fedra, Hernández-Aguilera Anna, Fort-Gallifa Isabel, Camps Jordi, Joven Jorge
Universitat Rovira i Virgili, Departament de Medicina i Cirurgia, Unitat de Recerca Biomèdica, 43201 Reus, Spain.
Unitat de Recerca Biomèdica, Hospital Universitari Sant Joan de Reus, Institut d'Investigació Sanitària Pere Virgili, 43201 Reus, Spain.
Antioxidants (Basel). 2019 Jul 10;8(7):213. doi: 10.3390/antiox8070213.
We investigated alterations in the levels of the antioxidant paraoxonase-1 (PON1) and the lipoprotein profile (analyzed by nuclear magnetic resonance) in patients with lung cancer (LC) or head and neck cancer (HNC), and the effects produced thereon by radiotherapy (RT). We included 33 patients with LC and 28 patients with HNC. Before irradiation, and one month after completion of RT, blood samples were obtained. The control group was composed of 50 healthy subjects. Patients had significantly lower serum PON1 activity and concentration before RT than the control group. PON1-related variables were good predictors of the presence of LC or HNC, with analytical sensitivities and specificities greater than 80%. Patients showed a significant increase in the number of particles of all subclasses of very-low-density lipoproteins (large, medium and small). However, these changes were not maintained when adjusted for age, sex, and other clinical and demographic variables. Irradiation was associated with a significant increase in PON1 concentration and, only in patients with HNC, with an increase in high-density lipoprotein-cholesterol concentration. Our results suggest that determinations of the levels of PON1-related variables may constitute good biomarkers for the evaluation of these diseases. Studies with a larger number of patients are needed to fully confirm this hypothesis.
我们研究了肺癌（LC）或头颈癌（HNC）患者体内抗氧化对氧磷酶-1（PON1）水平和脂蛋白谱（通过核磁共振分析）的变化，以及放疗（RT）对其产生的影响。我们纳入了33例LC患者和28例HNC患者。在放疗前及放疗结束后1个月采集血样。对照组由50名健康受试者组成。患者放疗前血清PON1活性和浓度显著低于对照组。与PON1相关的变量是LC或HNC存在的良好预测指标，分析敏感性和特异性均大于80%。患者极低密度脂蛋白所有亚类（大、中、小）的颗粒数量均显著增加。然而，在对年龄、性别以及其他临床和人口统计学变量进行校正后，这些变化未持续存在。放疗与PON1浓度显著升高相关，且仅在HNC患者中，与高密度脂蛋白胆固醇浓度升高相关。我们的结果表明，测定与PON1相关的变量水平可能是评估这些疾病的良好生物标志物。需要开展更多患者参与的研究来充分证实这一假设。
