Institute of Human Nutrition and Dietetics, Faculty of Food Sciences and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, Poland.
First Subdepartment of Pediatrics, Department of Pediatric Gastroenterology and Metabolism, Poznan University of Medical Sciences, Szpitalna 27/33, 60-572 Poznan, Poland.
Nutrients. 2019 Jul 10;11(7):1557. doi: 10.3390/nu11071557.
The hypothesis that habitual fat intake, the genotype, the Mediterranean diet or the central European diet for 16 weeks affect biomarkers of inflammation in centrally obese postmenopausal women, was tested in a randomized controlled trial. Dietary intake was assessed using a three-day food diary. Lipid parameters were measured using a Beckman Coulter AU analyzer. Transcription of and genes was analyzed in peripheral blood mononuclear cells using real-time PCR. Concentrations of tumor necrosis factor alpha (TNFα) and interleukin 6 (IL6) were measured with ELISA. rs1800795 polymorphism of was analyzed using hydrolyzing probes. Higher energy intake from fat was associated with higher IL6 levels ( < 0.05). Significantly ( < 0.01) lower total cholesterol (T-C) and low-density lipoprotein cholesterol (LDL-C) concentrations were observed in the GG rs1800795 genotype group. Both diets significantly ( < 0.001) decreased TNFα concentrations. Neither gene transcription levels nor blood IL6 concentrations were affected by them. Our findings confirm that habitual fat intake may affect inflammation. The rs1800795 polymorphism alone did not significantly affect body weight or body composition in aimed group, but C-allele carriers had higher levels of T-C and LDL-C. This polymorphism did not affect inflammation. Both diets may lead to a decrease in TNFα concentration.
该假设认为，习惯性脂肪摄入、基因型、地中海饮食或中欧饮食，持续 16 周会影响中心性肥胖绝经后妇女的炎症生物标志物，这在一项随机对照试验中得到了检验。采用三天食物日记评估膳食摄入量。使用贝克曼库尔特 AU 分析仪测量血脂参数。采用实时 PCR 分析外周血单个核细胞中 和 基因的转录。采用 ELISA 测量肿瘤坏死因子 alpha（TNFα）和白细胞介素 6（IL6）的浓度。采用水解探针分析 基因 rs1800795 多态性。脂肪的能量摄入越高，IL6 水平越高（ < 0.05）。GG 基因型 rs1800795 组的总胆固醇（T-C）和低密度脂蛋白胆固醇（LDL-C）浓度显著降低（ < 0.01）。两种饮食均显著降低 TNFα 浓度（ < 0.001）。两种饮食均未影响 和 基因的转录水平或血液中 IL6 浓度。我们的研究结果证实，习惯性脂肪摄入可能会影响炎症。rs1800795 多态性单独作用于目标人群时，对体重或身体成分没有显著影响，但 C 等位基因携带者的 T-C 和 LDL-C 水平更高。该多态性不影响炎症。两种饮食都可能导致 TNFα 浓度降低。
