Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki (A.U.Th), Thessaloniki, Greece.
Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki (A.U.Th), Thessaloniki, Greece.
Dent Mater. 2019 Sep;35(9):e220-e233. doi: 10.1016/j.dental.2019.06.003. Epub 2019 Jul 10.
This study aimed to develop silver nanoparticle (AgNP)-doped TiAlV alloy surfaces and investigate their antibacterial properties against representative periopathogens and potential cytotoxicity on osteoblastic cells.
AgNPs of different size distributions (5 and 30nm) were incorporated onto the TiAlV surfaces by electrochemical deposition, using colloid silver dispersions with increasing AgNP concentrations (100, 200 and 300ppm). The time-course silver release from the specimen surfaces to cell culture media was assessed by Atomic Absorption Spectroscopy (AAS). Cell attachment, viability and proliferation were investigated by SEM, live/dead staining MTT and BrdU assays. The antibacterial effects were assessed against P. gingivalis and P. intermedia by serial dilution spotting assays.
A time- and concentration-dependent silver release from the experimental surfaces was observed. Overall, cell viability and attachment on the AgNP-doped surfaces, suggested adequate cytocompatibility at all concentrations. A transient cytotoxic effect was detected at 24h for the 5nm-sized groups that fully recovered at later time-points, while no cytotoxicity was observed for the 30nm-sized groups. A statistically significant, concentration-dependent decrease in cell proliferation rates was induced at 48h in all AgNP groups, followed by recovery at 72h in the groups coated with 5nm-sized AgNPs. A statistically significant, concentration-dependent antibacterial effect up to 30% was confirmed against both periopathogens.
This study sheds light to the optimal size-related concentrations of AgNP-doped TiAlV surfaces to achieve antibacterial effects, without subsequent cytotoxicity. These results significantly contribute to the development of antibacterial surfaces for application in oral implantology.
本研究旨在开发载银纳米颗粒（AgNP）的 TiAlV 合金表面，并研究其对代表性牙周病原菌的抗菌性能以及对成骨细胞的潜在细胞毒性。
通过电化学沉积，使用胶体银分散体将不同粒径分布（5nm 和 30nm）的 AgNP 掺入 TiAlV 表面，AgNP 浓度逐渐增加（100、200 和 300ppm）。通过原子吸收光谱法（AAS）评估从试样表面到细胞培养介质的银释放时间过程。通过 SEM、活/死染色 MTT 和 BrdU 分析评估细胞附着、活力和增殖。通过连续稀释点样测定法评估对 P. gingivalis 和 P. intermedia 的抗菌效果。
观察到实验表面的银释放具有时间和浓度依赖性。总体而言，AgNP 掺杂表面上的细胞活力和附着表明在所有浓度下均具有足够的细胞相容性。在 24 小时时，观察到 5nm 大小的组出现短暂的细胞毒性效应，而在 30nm 大小的组中未观察到细胞毒性。在所有 AgNP 组中，48 小时时诱导了细胞增殖率呈浓度依赖性的显著下降，随后在涂覆有 5nm 大小的 AgNP 的组中在 72 小时时恢复。证实了对两种牙周病原菌具有浓度依赖性的抗菌作用，高达 30%。
本研究阐明了实现抗菌效果而无后续细胞毒性的最佳相关粒径浓度的 AgNP 掺杂 TiAlV 表面。这些结果为开发用于口腔种植学的抗菌表面做出了重要贡献。
