Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Int J Biol Macromol. 2019 Oct 1;138:755-763. doi: 10.1016/j.ijbiomac.2019.07.085. Epub 2019 Jul 12.
Molecular probes have become an important tool to decipher cell and molecular events, as well as contributing to clinical diagnosis. In this study, we report on a novel bioluminescence affiprobe and evaluate its functionality by monitoring HER2 expression in the prostate cancer cells. The proposed bioluminescence affiprobe consists of a binding domain, an HER2 specific affibody molecule and a bioluminescent domain, recombinant Renilla luciferase. The experiments indicate that bioluminescence affiprobe can serve as a reliable and user-friendly probe for the detection of human HER2 positive prostate cells as well as ex vivo based detection of HER2-positive human prostate cancer specimens using luminometeric-based assays.
分子探针已成为破译细胞和分子事件的重要工具，并有助于临床诊断。在这项研究中，我们报告了一种新型的生物发光亲和探针，并通过监测前列腺癌细胞中的 HER2 表达来评估其功能。所提出的生物发光亲和探针由一个结合域、一个 HER2 特异性亲和体分子和一个生物发光域、重组海肾荧光素酶组成。实验表明，生物发光亲和探针可以作为一种可靠且用户友好的探针，用于检测人 HER2 阳性前列腺细胞，以及使用基于发光计的测定法对 HER2 阳性人前列腺癌标本进行离体检测。
