Department of Pharmacology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey.
Department of Pharmacology, Faculty of Pharmacy, Binali Yıldırım University, 24100, Erzincan, Turkey.
Inflammopharmacology. 2019 Dec;27(6):1169-1178. doi: 10.1007/s10787-019-00617-1. Epub 2019 Jul 15.
This study aimed to investigate the role of the 5-HT receptor in fever mechanisms and its possible effect on the antipyretic mechanism of paracetamol.
The study consisted of eight experimental groups and one control group. Group I: healthy, II: LPS, III: LPS + PARA, IV: LPS + AGO, V: LPS + ANTA, VI: LPS + AGO + ANTA, VII: LPS + AGO + PARA, VIII: LPS + ANTA + PARA, and IX: LPS + AGO + ANTA + PARA. Rectal temperatures were measured with a rectal thermometer. At the end of the experiment, tissues were examined molecularly. Real-time PCR mRNA expression analyses were performed for the 5-HT7 receptor, IL-6, and TNF-α in hypothalamus tissue.
The mean differences in rectal temperature increased in the LPS, LPS + ANTA, and LPS + AGO + ANTA groups when compared to the healthy group and decreased in the LPS + PARA, LPS + AGO, LPS + AGO + PARA, and LPS + AGO + ANTA + PARA groups when compared to the healthy group. The IL-6 and TNF-α mRNA expression increased in the LPS, LPS + ANTA, and LPS + AGO + ANTA groups when compared to the healthy group in the 2nd and 4th hours. The IL-6 and TNF-α expression decreased in the LPS + PARA, LPS + AGO, LPS + AGO + PARA, and LPS + AGO + ANTA + PARA groups when compared to the LPS group in the 2nd and 4th hours. The 5-HT receptor mRNA expression increased in the LPS group when compared to the healthy group in the 2nd hour. The 5-HT receptor mRNA expression decreased in the LPS + AGO and LPS + AGO + PARA groups when compared to the LPS group in the 2nd hour. The 5-HT receptor mRNA expression increased the in LPS + ANTA and LPS + ANTA + PARA groups when compared to the LPS group in the 2nd hour.
The 5-HT receptor is a potential defense mechanism in stopping fever and the antipyretic property of paracetamol is not due to the 5-HT receptor.
本研究旨在探讨 5-HT 受体在发热机制中的作用及其对扑热息痛解热机制的可能影响。
研究包括八个实验组和一个对照组。I 组：健康组；II 组：LPS 组；III 组：LPS+PARA 组；IV 组：LPS+AGO 组；V 组：LPS+ANTA 组；VI 组：LPS+AGO+ANTA 组；VII 组：LPS+AGO+PARA 组；VIII 组：LPS+ANTA+PARA 组；IX 组：LPS+AGO+ANTA+PARA 组。采用直肠温度计测量直肠温度。实验结束时，对组织进行分子水平检查。对下丘脑组织中的 5-HT7 受体、IL-6 和 TNF-α进行实时 PCR mRNA 表达分析。
与健康组相比，LPS、LPS+ANTA 和 LPS+AGO+ANTA 组的直肠温度平均差异升高，而 LPS+PARA、LPS+AGO、LPS+AGO+PARA 和 LPS+AGO+ANTA+PARA 组的直肠温度平均差异降低。与健康组相比，LPS、LPS+ANTA 和 LPS+AGO+ANTA 组在第 2 小时和第 4 小时的 IL-6 和 TNF-αmRNA 表达增加。与 LPS 组相比，LPS+PARA、LPS+AGO、LPS+AGO+PARA 和 LPS+AGO+ANTA+PARA 组在第 2 小时和第 4 小时的 IL-6 和 TNF-α表达降低。与健康组相比，LPS 组在第 2 小时的 5-HT 受体 mRNA 表达增加。与 LPS 组相比，LPS+AGO 和 LPS+AGO+PARA 组在第 2 小时的 5-HT 受体 mRNA 表达降低。与 LPS 组相比，LPS+ANTA 和 LPS+ANTA+PARA 组在第 2 小时的 5-HT 受体 mRNA 表达增加。
5-HT 受体是阻止发热的潜在防御机制，扑热息痛的解热作用不是由于 5-HT 受体。
